EMAGE mouse gene expression spatial database

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EMAGE:32054

Galnt1 ( MGI:894693)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004959_01	euxassay_004959_02	euxassay_004959_03	euxassay_004959_04	euxassay_004959_05

euxassay_004959_06	euxassay_004959_07	euxassay_004959_08	euxassay_004959_09	euxassay_004959_10

euxassay_004959_11	euxassay_004959_12	euxassay_004959_13	euxassay_004959_14	euxassay_004959_15

euxassay_004959_16	euxassay_004959_17	euxassay_004959_18	euxassay_004959_19	euxassay_004959_20

euxassay_004959_21	euxassay_004959_22	euxassay_004959_23	euxassay_004959_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

mandible

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

palatal shelf

strong

regional

strong expression: see section 09 10 15 16 17

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18 19

clavicle

strong

regional

strong expression: see section 08 09 15 16

retina

moderate

regional

moderate expression: see section 01 02 03 22 23 24

trachea

moderate

regional

moderate expression: see section 12 13

thymus primordium

strong

regional

strong expression: see section 10 moderate expression: see section 11 12 13 14

telencephalon ventricular layer

strong

regional

strong expression: see section 21 22 23 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

maxilla

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 24

liver

strong

regional

strong expression: see section 01 02 moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

4th ventricle

strong

regional

strong expression: see section 21 moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

lower jaw incisor

strong

regional

strong expression: see section 11 14 15

upper jaw incisor

strong

regional

strong expression: see section 11 15

lung

strong

regional

strong expression: see section 02 moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 20 21 22 23 24

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7996
Entity Detected:	Galnt1, ( MGI:894693)
Sequence:	sense strand is shown >T7996 GACAAGAGGTAATCCGGCGCCTTCGTTTTCAGCCGCCCGCGACCGACTCGCCGAGGCTTTACTCGCTTGC CGCCGTCTTTGTCCGCACACAGATGATTTGAGACATGTGGATTTGTGATATGAATTCATTTGGAAAGCCA CTTTGGGGCTATGACTGTGATTTCTGATGAAACCAAATTGGAATAATTTTCATGATATTTGTATATTTAT ATCTATATATATTTTAAAATTTTACATTTGACTTAAAGTGCCATGAGAAAATTTGCATATTGCAAGGTGG TTCTAGCCACCTCCTTGGTTTGGGTACTCTTGGATATGTTCCTGCTGCTTTACTTCAGTGAATGCAACAA ATGTGAAGAGAAGCAGGAGAGAGGACTTCCTGCTGGTGACGTTCTAGAGCTAGTGCAGAAACCTCATGAA GGTCCTGGAGAAATGGGGAAACCAGTCGTCATTCCGAAAGAGGATCAAGAAAAGATGAAAGAGATGTTTA AAACCAATCAGTTCAACTTAATGGCAAGTGAGATGATTGCACTCAACAGATCCTTACCAGATGTGAGGTT AGAGGGGTGTAAAACAAAGGTGTATCCAGATAACCTTCCTACAACAAGTGTGGTGATTGTTTTCCACAAT GAGGCATGGAGCACACTTCTGCGAACAGTCCATAGTGTCATTAATCGCTCGCCAAGACATATGATAGAAG AGATTGTTCTTGTAGATGATGCCAGTGAAAGAGACTTTTTGAAAAGACCTCTAGAGAGTTATGTGAAAAA ACTGAAAGTGCCGGTTCATGTAATTCGAATGGAGCAGCGCTCTGGGTTGATCAGAGCTAGATTGAAAGGA GCTGCTGTGTCCAGAGGCCAAGTGATCACCTTCTTAGATGCTCACTGTGAATGCACCGCAGGGTGGCTGG AGCCTCTGTTGGCCCGGATCAAACATGACAGGAGGACAGTGGTGTGTCCTATCATCGACGTGATCAGTGA TGACACTTTCGAGTACATGGCGGGCTCTGACATGACCTATGGTGGCTTCAACTGGAAGCTCAATTTTCGT TGGTATCCTGTTCCCCAGCGAGAAATGGACAGACGGAAAGGTGACAGGACTCTTCCTGTCAGAACACCTA CAATGGCTGGAGGCCTTTTTTCAATAGACAGAGATTACTTTCAGGAAATTGGAACATACGATGCTGGAAT GGATATTTGGGGAGGAGAAAACCTAGAAATTTCATTTAGGATTTGGCAGTGTGGAGGAACTTTGGAAATA GTGACATGCTCCCATGTTGGTCATGTGTTTCGGAAAGCTACACCTTACACATTCCCAGGAGGGACAGGGC AGATTATCAATAAGAATAACAGGCGGCTTGCAGAAGTATGGATGGATGAATTCAAGAATTTCTTCTATAT AATTTCTCCAGGTGTTACAAAGGTAGATTATGGAGATATATCATCAAGACTTGGCCTAAGACGTAAACTA CAATGCAAACCTTTCTCTTGGTACCTAGAAAATATTTACCCTGATTCTCAAATTCCTCGTCACTATTTCT CATTGGGAGAGATACGAAATGTGGAAACAAATCAGTGTCTAGATAACATGGCTAGAAAAGAGAATGAAAA GGTTGGAATCTTTAATTGTCATGGTATGGGAGGTAATCAGGTTTTCTCCTACACTGCCAACAAAGAAATT AGAACAGACGACCTTTGTTTGGACGTTTCCAAACTTAATGGCCCAGTCACCATGCTCAAATGCCACCACC TAAAAGGCAACCAGCTCTGGGAGTATGACCCAGTGAAATTAACCCTGCAGCATGTGAACAGCAATCAGTG CCTGGATAAAGCCACAGAGGAGGACAGCCAGGTGCCCAGCATTAGAGACTGCACTGGAAGCCGGTCCCAG CAGTGGCTTCTTCGAAACGTCACCCTTCCAGAAATATTCTGAGACCAAATTTACAACAACAAAGAAAGAA GGAAGGATTGACTGGGCTACCTCAGCATACATTCTGCCACATTCTTCAGTAGCAAAAAAGGAAAGTGCTT CCCTCTTCTGTGGGATGTTAAGGTTTCTCAGCCATTAAAACTCAGACTCCTAGCTTTTCACTTGCTGTGA ACCGGCCTTCCTGTCCCAGGACGTGCGACTGCAAAGTAGCGAGACTGTGCACACTGATGTTTACAAGGCT GGAAGAGTCTTCATCAAGAGCAGCGGCAAAGCCTTCAGATCCCAAACAGGCGCACGAGCGCTCTCGGGAT AGATGCACTTTCTACAGCGTGGGTGCACGTCAGCCGTTTCTGCTGAATGTGCCTTGTGAAGAAGAGTTTT CCTGATTAGAATCAGTAGCTGGAATATTAAATATTGATAGAAAAGTAAGTGGAAAAGAGCTGGAACCAGG TTCAGAATCATGAAAACATTTTTACAACAATAAAAATTATGTCAAACAGGGTTTAAAGGAAATTAAAAAG AATTATGAGAAGTACAATTTGTTATAGTATAGTATGAGATTTCTATATAGATTTTATACCTCAGTGGGGA AAAACTGATTCCAGTGATGTTGATGTTGTTTTCATCTGTGATAGTCATGGATGCTTCTATTTTCCTCGGG GTGCTCAGAGTGAGATAGTGGGTGGGGTGAGCTCTCTGAACATAGTTTGAATTTCTTTTTACAATATTCT TATTTGGTTTGTGGGCTGTTGGAATTGTAAGTTTAATTGCCATTCTTTAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30019829 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30019829 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004959 same experiment
	EMAGE:31598 same embryo
	EMAGE:30662 same embryo
	EMAGE:30640 same embryo
	EMAGE:30986 same embryo
	EMAGE:31995 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source