EMAGE mouse gene expression spatial database

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EMAGE:31598

Fv1 ( MGI:95595)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005015_01	euxassay_005015_02	euxassay_005015_03	euxassay_005015_04	euxassay_005015_05

euxassay_005015_06	euxassay_005015_07	euxassay_005015_08	euxassay_005015_09	euxassay_005015_10

euxassay_005015_11	euxassay_005015_12	euxassay_005015_13	euxassay_005015_14	euxassay_005015_15

euxassay_005015_16	euxassay_005015_17	euxassay_005015_18	euxassay_005015_19	euxassay_005015_20

euxassay_005015_21	euxassay_005015_22	euxassay_005015_23	euxassay_005015_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 09 15

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06

trigeminal v nerve

moderate

regional

moderate expression: see section 08 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12

thyroid gland

moderate

regional

moderate expression: see section 10 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 15 weak expression: see section 08 14

adenohypophysis

moderate

regional

moderate expression: see section 09 10 13 14 weak expression: see section 11 12

submandibular gland primordium

moderate

regional

moderate expression: see section 06 15 16 weak expression: see section 07 08 09

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 16

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 13 14 weak expression: see section 12

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 19 20 21 weak expression: see section 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 14

vibrissa

moderate

regional

moderate expression: see section 03 04 05 06 07 08 20 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8057
Entity Detected:	Fv1, ( MGI:95595)
Sequence:	sense strand is shown >T8057 AAGAGGTAATCCCGAACTGGAATTTGGATCCGAGGCTTGTAGGACGCAGCAGCCGAGTTCTAGGGAAACA AAAGTCTGAAGATGAATTTCCCACGTGCGCTTGCTGGTTTCTCGAGCTGGCTCTTCAAACCTGAACTTGC CGAGGACTCTCCGGATAATGACTCTCCGGATAATGACACTGTTAACCCATGGCGTGAGCTGCTGCAGAAG ATAAATGTGGCCGATCTCCCCGATTCATCCTTTTCGAGCGGTAAGGAACTTAATGACTCTGTGTACCATA CTTTTGAACATTTCTGCAAGATTAGGGACTATGACGCAGTTGGCGAGCTGCTTCTGGCATTTCTGGATAA AGTAACAAAGGAAAGGGACCAATTCAGAGATGAAATTTCCCAGCTCCGAATGCACATAAATGATCTAAAG GCTTCTAAGTGTGTCCTGGGGGAGACTCTTCTTTCCTACCGCCACAGGATTGAAGTTGGGGAAAAACAGA CTGAAGCCCTCATTGTGAGGTTAGCTGATGTGCAATCCCAGGTCATGTGTCAGCCTGCCCGGAAAGTGTC TGCAGATAAGGTGAGGGCACTGATTGGTAAAGAATGGGATCCGGTAACCTGGGATGGAGATGTGTGGGAG GACATAGATTCTGAAGGGTCTGAGGAAGCTGAGTTGCCCACTGTCTTGGCCTCTCCATCCTTGTCTGAGG AAAGTGGTTATGCCTTGTCTAAAGAACGCACCCAGCAGGACAAAGCAGATGCCCCTCAGATCCAGTCTTC AACATCCTTAGTTACTTCTGAACCTGTCACCAGACCCAAGTCTCTGTCTGACCTTACAAGTCAGAAACAC CGCCATACTAATCATGAACTCAATTCACTTGCTCACTCAAATCGCCAAAAGGCAAAGGAACATGCTAGGA AATGGATTTTAAGGGTGTGGGATAATGGTGGGAGGCTCACAATACTGGATCAGATTGAATTTCTCAGTTT AGGTCCTTTGAGCCTTGATAGTGAGTTTAATGTCATAGCCCGCACTGTTGAAGATAATGGTGTGAAGAGT TTGTTTGATTGGTTGGCTGAAGCATGGGTCCAGAGATGGCCTACTACAAGAGAGCTGCAGTCGCCTGACA CCCTGGAGTGGTATTCTATTGAGGATGGGATTAAAAGGCTTAGGGAACTTGGAATGATAGAGTGGCTTTG TGTAAAAGCTACTTGTCCACAGTGGAGGGGCCCGGAAGATGTACCCATCACGAGAGCTATGAGGATAACT TTTGTCCGGGAAACTGTAGAGACTTGGAAGAGCTTTGTATTTAGCCTCCTCTGTATAAAGGACATAACAG TGGGGAGCGTGGCTGCTCAGTTGCATGATCTAATAGAATTAAGTTTAAAGCCAACAGCAGCTACAAAACT CTGAACTCACCAAGATAGTATAGATGATACTTATCCAAAGGTTGCCAAATACAAACAAACTGGACTTTTT AAATGTCTTACCTAATCATTTTCATAATTGTTCCTACTTTATGTTGTTTATTGCTGTAAGAGAATGACTT TTTATGTACACAAAAAGGAGGAAATGTATAGGGTTGGTCTGGTGCTGCTTGTATTCAAATGCTAAATCCT GTCTGGGTTGCTCCAAGATGAGCCGTTCGATCTTCATGTATACCAGCCTTTGTTGTATAAACCTTGCTCC CCAATTTAAAACTATTGATTAGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTGCTCTTCTGA AGATCCTGAGTTCAAATCCCAGCAACCACATGGTGACTCACTGTTGGGAGCCGCGCCCACATTCGCCGTT ACAAGATGGCGCTGACAGCTGTGTTCTAAGTGGTAAACAAATAATCTGCGCATGTGCCAAGGGTATCTTA TGACTACTTGTGCTCTGCCTTCCCCGTGACGTCAACTCGGCCGATGGGCTGCAGCCAATCAGGGAGTGAC ACGTCCGAGGCGAAGGAGAATGCTCCTTAAGAGGGACGGGGTTTCGTTTTCTCTCTCTCTTGCTTCTTGC TCTCTTGCTTCTTACACGCTTGCTCCTGAAGATGTAAGAAATAAAGCTTTGCCGCAGAAGATTCTGGTCT GTGGTGTTCTTCCTGGCCGGTCGTGAGAACGCGTCTAATAACAATTGGTGCCGAATTCCGGGACGAGAAA AAGCTCGGGACTGGCGCAAGGAAGATCCCTCATTCCAGAACCAGAACTGCGGGTCGCGGTAATAAAGGTT CCCGTAAAGCAGACTGTTAAGAAGGATTCAACTGTATGAATTCAGAACTTTTCAGCTGGGGAACGAGAGT ACCAGTGAGTACAGCTTTACGAGGTAAGTCTGATCTTGAACTTTCTAACGAAATTCAAGACAGTCTATCA GAAGTAAAGTGGAATATGTTTGGCCTTGAATTTTTTCTGGTGTTAGGAGCCCTTTTGTTCCTTTTCACAT GTTATCAAGTGATTAAGATAGGGCGGATTCTAGAGGAAATTCAGGACAAGCTATCAGAAGTAAAGCGGGG AGAGAGAGTAGGAACAAAGAGGAAATATGGTACACAAAATAAGTATACAGGCCTTTCCAAGGGTCTTGAA CCCGAGGAAAAGTTAAGGTTAGGTAGGAATACCTGGAGAGAGATTAGAAGAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30060270 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30060270 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005015 same experiment
	EMAGE:32054 same embryo
	EMAGE:30662 same embryo
	EMAGE:30640 same embryo
	EMAGE:30986 same embryo
	EMAGE:31995 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source