EMAGE mouse gene expression spatial database

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EMAGE:31995

BC052883 ( MGI:3027899)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8055
Entity Detected:	BC052883, ( MGI:3027899)
Sequence:	sense strand is shown >T8055 AAGAGGTAATCCGAAGTGGCAAGTGATGTGACGCGAGGAAGGGAGAACTGACCTACTCGGAGGCGGTGGG GAAAGGATCAGATCACCTGGTTCACTGGCTTGTAACCAAGAACCTGCTCCTGGGCTCAGAATTTCAGCTG ATGGTGACTTCTCTGTTGGTAACCTGTGCCTTCCTTTCCCTTGGATGGTTTTTTATGGCCACCCGTGGAA CGCTGAGCCTCTGACAAAGATTTATAGTGTTTTCCACTCCGAGACCTCCTTGTCTGCACTGAGATGGCCG ACACAGAGACTGACCAGGGACTGAACAAGAAATTAAGCTTCTCCTTTTGTGAAGAAGACACTGAGAGTGA AGGGCAGATGACTGCACAAGACATCGGAGGGGCCCAGAGCCAAAAGCCTGGGAGGGAAGAAAGTGAGGAG TTGGAGGGTCCCGTGCCTCCTACCAGAGATGAACTTCATACTTCTCTGAGCAGAGACAAGGGAAGTCCAG GTCCTGATCTTTGGAAATCAGTTTCAGGCCCTCCCAAATGCCCAGAGACACCAGTATTACACAGCAAGAG CAAGCCGCCAGTCCCTACCAACTTCTCCACTCCCAAAAATTCGCTGGGTCAGCCAGAGATTTCTCTGCTA GAGAAGCTCTCCTCCCGAAGCTCTAGACATATGAGGCTCACACCTGCTTCCCTTGTGGATGAGAAGACCT CTTTGTCTCTGGTCAATATCAACCCATTCACTCCGGAGACCTACAGAAAATTACTCCTTCAATCAAAGGG CAAGAGGAAGACCAGAGACTATCTTGAGGAAACTGGAGAAGAGGAAAGCAAATCAGTACAGTGGCTGCCT GCTAAGAGAAGTATTCTACAAGAAACTAATATGGCTTCCCGCTATGAAAAAGAATTCTTCGAGATAGAAA AAATCGGGGTTGGAGAATTTGGGACAGTCTACAAGTGCATTAAGAGGCTGGATGGATGCATTTATGCAAT AAAGCGCTCTGCAAAGTCCTTTTCAGGATTATCAAATGAATTGGATTTGCATGAAGTTTATGCTCATGCA GTGCTTGGCCATCATCCCCATGTGGTACGCTACTACTCATCATGGATAGAAGATGATCATGTGGTCATTC AAAATGAATACTGTAATGGTGGGAGCCTGCAAGCTGCTATATCTGAAAACACTGCGTCCAATAATCACTT CCAAGAGCCCAAACTCAAAGACATCCTTCTGCAGATTTCCTTGGGACTTAAGTACATCCACAATTCTGGC ATGGTACACCTGGACATCAAACCCAGTAACATCTTCATTTGTCATAAGATGCAGTGTGACTCTCCTGTAG GCCCAGAAGAGGCTGAAAGTGAAGCTGACTGGTTTCTCAATGCCAGTGTGATGTATAAAATTGGTGACCT GGGCCACGCGACATCAATCAGCAAACCTAAAGTGGAAGAAGGAGATACTCGTTTCCTGGCTAATGAGATC TTGCAAGAGAATTACCAACACCTCCCCAAAGCAGACATATTTGCCTTAGGATTAACAATCGCAGTGGCTG CAGGAGCAGAGTCTTTACCCATCAATGGTGACATGTGGCATCATATCCGCAAAGGGAACTTTCCAGAGAT TTCCCAAGAGCTCTCGGATGACTTTTATGGTCTACTCAAGAACATGATCCACCCAGCTCCAAAGGAGAGA CCATCTGCAGCAGCTCTGGCCAGAAGTCGAATTCTCTGGCCTTTCTTGGAGAAGACTGATGAACTCCAGA AACAACTGAATTTGGAGAAATCCAAGACAGCCACACTGAAAAGGGAACTGAAAAAAGCTCGGCATATCCA GACCCCTCAGAGAGAAGTCCACCATTGTTATTATCAGATCTGCAAAGGCTCAAGACACCTCCTGGTGGGA GGAAGGAGGAAGGCGCCCTCAAGCTTCACCCGTGGGACATCCTCCGTGTAGGAACTCACTTCTGGCCTTT TGCTACACAACACAGCCTTTTGAAAACTCTTACAGGATACAAAACTGAACCAGCTTGAATGTACAAAGAT CTCAAAAGCCATCCTGATGTGGCATCTATAGGTAAGATCACCGAGCTTCCAGGGTTTCTCCTTAGCAACG GTCTTGTGTATCGTAAGTACTGGAGTCTTCTTTTCCCTAGTGATGTATTTCTAGAGCTGCTTACTAAGGA CTCCTAATGAATAATCAGAAAACATGGCTCTGAACTGAAAGGAGAAGCAGAAATGTGCTACTTTGGCCTA TGGATTAAGAAGCTGCCGTTACTTATTCTTCACTGGGGTCCAAAGAGCTTTCCCTGTACATAAAAAGGGA TAAAATATGGCTTTAAGTGCCAGCTTCCAAGAGAATGTTGCCTGTATTTCAGAGTGCTTTCCAAGATCTG TCTGCTCCTCCAATCACCAGCTCCTTTGAGCAAGGAATTTGCCTACTATACTTTCTGAATGGAAGCAATC TCTTTTCTGATTTTGCATTATTAAATAAAAGACTATCATTGTGTGAAACAACTAGAGTAAGACTATAATT TGGACATTCCTGAGCTGCTTATTAATTTGTGAAACACAATCAGCAGAGGCAGAGGACCCAGGAATTTTGG TGTACCTGTTCCCCGTGTGTTCCTCACGTCTTAGGTCTCTGTGGCTGACTCCAGTTGCTTTTCAGTTTTG AACATCATAGCATCATCTGGTTCTCTTGATTTTTTTTTTTTACACCAGAACTCATCCTTTTGTTTTCTTG CTTTTTAACATATTTTAATCCAGATCATTGTAAATAAAGATTTTATCTTATCCAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30053490 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30053490 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004978 same experiment
	EMAGE:32054 same embryo
	EMAGE:31598 same embryo
	EMAGE:30662 same embryo
	EMAGE:30640 same embryo
	EMAGE:30986 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source