EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8174

Zik1 zinc finger protein interacting with K protein 1 ( MGI:108070)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005254. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005254_01	euxassay_005254_02	euxassay_005254_03	euxassay_005254_04

euxassay_005254_05	euxassay_005254_06	euxassay_005254_07	euxassay_005254_08	euxassay_005254_09

euxassay_005254_10	euxassay_005254_11	euxassay_005254_12	euxassay_005254_13	euxassay_005254_14

euxassay_005254_15	euxassay_005254_16	euxassay_005254_17	euxassay_005254_18	euxassay_005254_19

euxassay_005254_20	euxassay_005254_21	euxassay_005254_22	euxassay_005254_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8174_wholemount_strong.wlz

8174_wholemount_moderate.wlz

8174_wholemount_weak.wlz

8174_wholemount_possible.wlz

8174_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8174_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

medulla oblongata

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

metencephalon

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 moderate expression: see section 07

midbrain lateral wall

strong

regional

strong expression: see section 09 10 11 moderate expression: see section 12 13 14 15 16

spinal cord lateral wall

strong

regional

strong expression: see section 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9100
Entity Detected:	Zik1, zinc finger protein interacting with K protein 1 ( MGI:108070)
Sequence:	sense strand is shown >T9100 TCCAGATCGTGTGCAAGACCCCTTTGTCCAGAGGTGTACGGATCGAAGAATCTCAAAGGAGCGGTTCTAA GGGCGTAATATTCATCGCAGCCCCGGCACTGACAGTGGCAGTATTGTCCAGGTCCCTCGTCCCCGTTTCC CCGTTGTCTGCGCTCGCTTACTTGGATGGCTGTGCCCACGCTCATGCACCAGGCTTAGGTGTCCATGACT GCAGAAATGGATATGGCCCTCATGCAGGGCTGTGTGACCTTTCAAGATGTGGCCATTTGCTTCTCCCATG AAGAATGGAGACTTCTTGATGAGACTCAGAGGCTCCTGTACCTCAGTGTTATGCTGCAGAACTTTGCACT CATAAACTCCCAGGGTTGTGGGCATAAAACAGAAGATGAAGAGCGAAGGGTTTCTACACGAGCGTCAAAG GGTTTGAGATCCGAGACCACACCGAAAACTAACCTCTGTGAAAAGTGTGTCCCGATTCTGCAAGACATTT TGTGCCTGCCTGGTCTACCTGGGCAGAAACATAGTACAGAGGCAAGTTCAAAAGTTGATCAGCACCAAGA CCATAATAGTACAGGAAAACCCTTGGAGAAGAATGCTGACAGGTCTTCATACCTGTTCTACCTGTCAGCA AAGTCCTTCCCCAGTTGGGATGTTGAAAAAGACCTCCCAGATATACTGAGCCTTCTGAAGTCTCAGGTCT GTCCCAAAACTAAGAAGTACAGAAAAAGCACTGAGGGTAGGAAAGAAACAAGTCATGAAAGTGACAAGTC AGAAGAGTGTCAGAGCCTTTCCAGTCAAAAGCAAACACTTGCTCACCACCCAAAAACCTCCAATGGAAAG AAACTTTATGAGTGCAGCAAATGTGGAAAGACCTTCCGTGGCAAATACTCCCTTGATCAACATCAGAGAG TCCACACCGGAGAGAGGCCTTGGGAGTGTAGAGATTGTGGGAAATTCTTTAGCCAGACCTCCCACCTGAA TGATCACCGTAGAATACACACTGGAGAGAGGCCTTATGAGTGCAGTGAATGTGGGAAATTATTTAGACAG AACTCCAGTCTTGTTGATCACCAGAAAACTCATACTGGAGCAAGACCATATGAGTGTAGCCAGTGTGGGA AGTCCTTTAGTCAAAAAGCTACACTTGTTAAACACAAGAGAGTTCACACTGGAGAAAGGCCTTACAAGTG TAGTGAGTGTGGGAATTCCTTTAGTCAAAGTGCCATTCTTAACCAACACCGGAGAATTCACACTGGAGTA AAGCCTTATGAATGTAGAGAATGTGGGAAATCTTTTAGCCAAAAAGCTACCCTTATTAAACACCAGCGAG TTCACACTGGAGAAAGGCCTTACAAGTGTAGTGAGTGTGGGAAATCTTTCAGTCAAAGCTCCATCCTTAT TCAACACCGGAGAATTCATACAGGAGCAAGGCCCTATGAGTGTAGCCAGTGTGGAAAGTCCTTTAGCCAA AAGTCCGGTCTCATTCAGCATCAGGTAGTTCACACTGGAGAAAGGCCATATGAATGTGACACATGTGGGA ATTCTTTTAGTCAGTGCTCTAGCCTCATTCATCACCAAAAATGTCATAACGCTTAAGAAACCTCATGAAA GCAGCAAATGTGGATAAGCCTTCAGCTGAAGGCCTAACATCATTTAGCTCCTCCAAATCCGTACTTGAGA AAAAGCCTTAGATTTATAGGGAATGCCCTCTTTTTGGTTTTGCTTTTGTTTTTTGTTTTTTAAAGTACTA TGACACTAGAACCTTTGCAGAGGATCCAACTACAATGTAAGGTTGATCTTCCAGTATCCAGGAATCAGAT TCCTCAGGAATTCAGGCACAAGTAGGTTCAGCAACTGCTTTGCCCATCCTGAACTGGCCAAGACCTTTGC CATTTTTGTACCAGTGTTTGTGGTAGAAGCTATCAGTCGTTCTCTACCACATGACCAGTGTGCAATGATT ACACCATCATGGTAAGGAAAGGCAAACTGCAGATTGCCTGGAAGAATTCTTCAATGGAGGATTTATGGAA AATTGGTCCCCCAGTTTTAACTGATTAAAATGTGGATATGACTAAGATTTCACCAAGAAGATCAAATTTA TTGTCTGCTAAAGATGTCTAGAATTTGCTTCCCTGCTTCTTTTAAAAAGTAGCTTTTAATCTGTGTCACA TGACAGTTAGGTGAATGTTTCCAGCCTTCTGATTATCAGTTGGTCACTGCCTTCTCCCCGTTGCTCTGAT TTGTACACTGATAAATGCCTTATCCCTAAAGCTACCTTACTCTTAAGAGTGCAGTTTACTAAGTGGGAAG GCTGGTAAACAGACTGATCATTTTAGGAAAGAATGGAAAGCTTTTATAGAGGGTCTCAACTTGGTTTACC TCTGCACGGACAGCAAAATAAAGAAAGCAAATTTGTATTGCTTCCCAGGCCAACAGCTGGGACTTCTTTA TTATAGTTTATGAATAACTGAATAAGTATTCATGTACATGAAGATCTAGTTGAAAACTTTCTGTGATCAT CTCAAGAAGATGTCTGTTATTTCCATAAGCTCCTTTACTTGTGGAAATGAAATGAATTGGCTCATTTCTT TTTCTCAAAGGATGTCCTAAAAATGCCTATGATGCAGAGAGACAGCTCATAAAGCTGTATAAAGAGTGTG CCTGGATAGGAATTATTCCATAGGCACCAGCCAATAAGATGACCACTCATGAGGTGATCAGAGCTAGATG TGAATCATCATTGGCATACAAAAGATGGGTATAGTGGAGTCTTCAGCCAGCCAGATGGAATGTGACTCTT TTAAAAGTGCACACAATCTTTGTCTTTGGCTGAATTCACAGGACTTAAAGATCTTTGTATTTACAATAGC TGAGGTCATTGTCAAAAATGGTATGGTTTTGTGGGATAGTGGAGTTTAACTTGATCATTTACCTCCAGTC TTGTTATAGAGATAGGAAAATAAGCAAATGAAGATTCTTTAGTCTTAGAATGTAGCTGTTATAATTGCAT TCCAGCTGCTGTGTTTGCAGTCTGGTATTCACAAATGTCTCTGCCATTTCCTGCATTAACAAAAGGCTGC CCTTATCTGTATATGAGGACAGAGTTCAATCAAAATGGGGGATACCAAGCCTTCTGGTTTGCAGAAATTA GGAGTTCTGAGCTTTGTACTTTGAGTCACTTTTTATAAAGTTTATCAATGTTTTAGTGAAATGCAGTCAA CTCTGATTTATCCTTGAAAAAAATCTTAAAATCATCTTTGTGATGATGGACTTGTCTGTGACTTACCTCA CCCTGTTAAGATCTCCCTTGTGTTCTCGGACTTGTAAGTGTTGAATAGCTTATATTTGGCTCTTAAGAGT GCAGTTTGTGAATGAAAACAAAAGGTTTTTTTTCCTTAGATACAGTGTCTTTTAGGAATACATAATTATT TTGAGATTCATCACTGCTGCTTTTTAATAACTTTATAGTGCTATACCATGTTTGGTTTTGTAACTATACA ACAATTTATTGATCTTTTTTATTCATTCGGCTCTTGGTTGAATCCTTTCTTGAGCTGTTACATATTATGG ATATGAGCATTTGATTCAATATGCTTACATTTTTAAATTCCTCTCGAGCCAGTAATTAAGTATATGTGAA TGCTTCTATTTTTAAGAAAGTGTCAAACTGGTATAGATTGCTCTAGATCAGTGGTTCTCAACCTTTCTGA TGCTGCAATTCTTTAATACAGTTCCTCATGCTGTGGTAACCCTCAACCATAGAATTATTTTTGTTGCTAC TTCACAACTGTAATTTTGCTGCTGGTATGAATTGTAAATATTTGTGTTTTCCTATGGTCTTAGGTGACAC CTGTGAAAGGGTCATTTGACTCCCAAAAGGGTCACGACCCATTGAGAACAACTGATGTAGACAGTGTATG AACATTCTTGCTAGTACTTGTGTGGTTAATGAGTTTGCTTTGAGCCATTTGAATAGCTGTATAATAACAC ACTGTATAGTCTTAATTGCTTTTTTCGTGTTTCATGATACTGAATATTTTTGTGTGTTTACTTGCCAAGT GTATTTTTTAAAACTTGTTTTTTTGCCTATTTTCAAGTGAAAATTCATGTTACTGTCCTTTTCATTAATA AATAGATGTACACTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6821378 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6821378 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8178 same embryo
	EMAGE:8173 same embryo
	EMAGE:8177 same embryo
	EMAGE:8176 same embryo
	EMAGE:8175 same embryo
	EurExpress:euxassay_005254 same experiment
	MGI:4829355 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source