EMAGE mouse gene expression spatial database

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EMAGE:8175

Vstm2l V-set and transmembrane domain containing 2-like ( MGI:2685537)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007444. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007444_01	euxassay_007444_02	euxassay_007444_03	euxassay_007444_04

euxassay_007444_05	euxassay_007444_06	euxassay_007444_07	euxassay_007444_08	euxassay_007444_09

euxassay_007444_10	euxassay_007444_11	euxassay_007444_12	euxassay_007444_13	euxassay_007444_14

euxassay_007444_15	euxassay_007444_16	euxassay_007444_17	euxassay_007444_18	euxassay_007444_19

euxassay_007444_20	euxassay_007444_21	euxassay_007444_22	euxassay_007444_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8175_wholemount_strong.wlz

8175_wholemount_moderate.wlz

8175_wholemount_weak.wlz

8175_wholemount_possible.wlz

8175_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8175_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9086
Entity Detected:	Vstm2l, V-set and transmembrane domain containing 2-like ( MGI:2685537)
Sequence:	sense strand is shown >T9086 GGAGCCCCGATGTGAGGCGGCGGCGCCCCCGGCCGGAGCGCGCAGCATGGGGGCCCCGCTGGCCGCGGCG CTGGGCGCCCTCCACTACCTGGCACTTTTCCTGCAACTCGGCGGCGCCACGCGACCCGCAGGCCATGCGC CCTGGGACAACCACGTCTCCGGACACGCACTCTTCACAGAGACACCCCATGACATGACGGCACGGACAGG CGAGGATGTAGAGATGGCCTGCTCCTTCCGAGGCAGTGGTTCCCCATCATACTCACTGGAGATTCAGTGG TGGTACCTGCGCAGCCACCGGGACTGGACTGACAAGCAGACGTGGGCCTCCAACCAGCTCAAAGCTTCCC AGCAGGAGGACTCGGGGAAGGACGCCACCAAAATAAGTGTGGTCAAGGTGGTGGGCAGCAACATCTCCCA CAAGCTTCGGCTGTCCCGGGTGAAGCCCACGGACGAAGGCACCTACGAGTGCCGCGTGATCGACTTCAGC GACGGCGGCAGAGGAGTCCCGCGTGTGCTTTGCTTGCTTATCCCCCTCCCGGCTCCGCCTCGTGCACCCC GTCCTCGCGGCCAGCCCCCTGGTGAGGAGCCGGGCCGGGGCCCAACGCTCCTGTTCCTCATCATTCTACC TGGAACCGGCTCCGGAACCCCGAGGGAGGCGGAGCCCCACCAGCCCCACGCGGGAGGCTGCCCCGCCAGG CAGAGTTAGCACCAGCAGGCTCGCTCAGGGCCCCCCGAGGCTCTCCCCGAGCCCCCCTCTGCCGGCAACC CCCCCCCACGCCCAGCCCCTTCTCGGGCCTGTGAAGTCCACCCAAGGGACCCCCTCCGCCCATCCCATCC CTATTCCTGATCAGGGGACACCCACCCTGGTGGTTTGTAAATATTTCTATTGGGCCCACCCCGCCCCGGA ACTGGCTCAAACCTCTGGCCACCCTCGGTGATGGAGGGGAGGTGGGAGGCCCAGGGCTTTGCCTAGGGGT GAGCGGGCCCTGTGTATATGACCCAACCTGTGAGTACCAGCCAACTCCAATAAAGCGGTTTAAACCAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6820223 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6820223 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8178 same embryo
	EMAGE:8173 same embryo
	EMAGE:8177 same embryo
	EMAGE:8174 same embryo
	EMAGE:8176 same embryo
	EurExpress:euxassay_007444 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source