EMAGE mouse gene expression spatial database

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EMAGE:8178

4933411K20Rik RIKEN cDNA 4933411K20 gene ( MGI:1914006)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005245. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005245_01	euxassay_005245_02	euxassay_005245_03	euxassay_005245_04

euxassay_005245_05	euxassay_005245_06	euxassay_005245_07	euxassay_005245_08	euxassay_005245_09

euxassay_005245_10	euxassay_005245_11	euxassay_005245_12	euxassay_005245_13	euxassay_005245_14

euxassay_005245_15	euxassay_005245_16	euxassay_005245_17	euxassay_005245_18	euxassay_005245_19

euxassay_005245_20	euxassay_005245_21	euxassay_005245_22	euxassay_005245_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8178_wholemount_strong.wlz

8178_wholemount_moderate.wlz

8178_wholemount_weak.wlz

8178_wholemount_possible.wlz

8178_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8178_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9108
Entity Detected:	4933411K20Rik, RIKEN cDNA 4933411K20 gene ( MGI:1914006)
Sequence:	sense strand is shown >T9108 CGCAGATACACTTTTGGCGTCATGGATCGGTTTGGAGATATCTCAGAAGGTGAAGTAGACCACTCTTTCT TTGACAGTGATTTTGAAGATGCAAAGAAATGTGAAAGTAATTCCATTTTTGACAAGCAAAATGATGATGA CCTTAAGGAAGGAATAAATAAGGACACCAAAAATGTAAACTTGAAATTTGGAGTACAAAATGATCATCTT AAGGAGAAAATAGATAATAACACAGAAAATGTGAACTTGAAACTTGGGCTACAAACAACAGAAAATTACC TTACTCAGAAAGGAAATGAAAGGAAAGCAAACTTTTCTTCAAAGGAACAACATATAGAAAATGATCCTAC ACAAGCACGAAGTTCCTCAGTGTTGACTTCTTCACGATCAAAAAAATCGTGTGATGCCACAAAAGGACAT AAATTAAACTTGCCCGTTCCAGATAGAATTCCTAAGATTGTGAAAGGTGAGGACGATTACTACACGGATG GGGAGGAAAGCAGCGACGATGGGAAGAAGTACGTGAGGTCTAAGTCAGCCAAGCCCTCTAGCAACTTAAA AAAAAACGTGAGTAAGAAGTATTCCTCCTCCTCCTTGTCTTCCTCATCTTCAAGATCAAACTCAGACTGC TCAGATATGGGGTCTGACAGGCAAAGGAGATCTGAGTCACACTCATCAGGGAAGTGTGTTTCTAGTGTAA CCCCCTCATCACCAAAACAGAGGTGTAAGTCAGGAAGAAAATCAAGTGCACAGCCTTCCAGCACTAAACA AAAGACTGGTGACTATCATGAGTCCGAAGGCAATGTACCAGACATAACTCCTTTGTCAACTCCAGATGTC AGCCCTGCCCAGTCGCTTGAGTTGGGCCAACCGCCTGATCAAAAAGTAAAAGTTAAAAAACAAGAAAATG TGAGCCGGGATGTATATGAGGATGCAGAGGCTTTAAAGAATGATTCAAGATGTGTGAAATCAGCCAAAAG GAAAGAAAAGCATGGACAGAGTTTTGCTCCAAAATCTTCAGTGTTAGATGCAAATCTAGACCGTAGATCT AAACAAAAAGTCTTACATGACACAATGGACCTAAATCATCTGTTGAAAGCTTTCCTGCAGTTAGATAAAA AGGGACCACAGAAACATCACTTTGAGCAGCCTGCAATAATACCTAGGAAGAACTACTCTTTCACAAGAGA AGAGGTGAGACAGATTGACCGGGAAAATCAGAGGCTTTTGAAAGAACTGTCAAGACAGGCTGAAAAACCA GGAAGCAAAAGTACAATTCCCGGAAGATCGATCGGCCATCCCCCTAAGTTGTATCATAGTGCTCTCAACA GACAGAGGGAACAACAAAGGATCGAAAGAGAAAATATGGCTTTATTGAAACGACTTGAAGCTGTGAAGCC AACAGTTGGCATGAAACGCTCTGAACAGCTGATGGACTACCATCGGAATATGAGTTACCTCAATCCTTCA CCGTCCGTCCGACGAGTGAGATCTACTCTTGGCCATTATAGCCCACTGAGAGGAGCTTCCAGGACATCCA GTGCAACCAGTGGTCTCAGCTGTAAGACTGACCGATCAGTGTTGGACACATCCAATGGCTTTTTGCTAAG ACCGAAACCCCCGAATGTTCGTACTGCTTGGTTGTAAAACCTTTTTTTTTATGATATACATTGTTCATCT GTTTGTCCTATTTTAACAATGTATTTTTCTGTACTACTATAACTATTTGCAAACATCTGTAATACTTTTT TTCAACAATTTATGCAACAATGAGTATAATTTATTGTTGTTCAGTGCAAATTGTTATCAAAAGACACCTT GCTGTAAAATCAGTGTTTGATACGGTGGCTGGCGTATTTATAGGAGCTGTATGTCTGTTTAATAGAGAAG AGGTTTGTTACATGTGACTATTTCTTCAATATAGAAACTCAGTTGTGCTGACAAAGCAGCAGTGGTGACA ATCTGTGTTGAAGCCAAGACACTCACTATATAGCTCTTAATCTATGTGATTTTCAGTTATCATAAGCGCT AAACACGGAGCTTGCCTTGTAAACGTGCACCTGTGCTTCTGTCTGCTCTACGTTGTCTGTAACTCGAGTC CTCACGTGCTTATGTGAGTGTTAGACTCCTTATGAAAACGCTTTGACGTCTGCTTCCATGGAGAAGCTGA CTTAGATTTGATGAGGAGGTGAGGAGCTTGTACTGTGCAATCCCTGATTCACCAAAGACACTTCTGAAGA GTCTGACTCTCCACCCACACCATCTCACACTGTGAGTAACAGGGTTCACTTCAGAAGCTGCTCTCTATGC CGTCTGGTAAAATACTCTTAAGGATTCTGAGGCAAGAGGTAGGCTTGAACAGCCTCATGTCTTGTGAGTT GTGACTGGTTTGTATCCAGATCACTTGCATAATAAACAAAAGCAAGTATTTGTTGGATCAGTGAGCAACA CTCTGAGCTTGAAGAACCAGAAGCGCATGCCTAGAGTATGTTAGAGCAGTGTTGGAAATGACACTGTTAG TCACTGTGTGTGCTGCTGTTGTGTATTTATTGTAGTAATAGAAGGCTTAGAGCAAGGAAGAAATGCTCTA GAGAAACCAAATAGAAACAAGAAAGAAAGTAATCACACATTGCCTAAAGCTATTGCCTTGATGCTAGAGA ATTCCCTTGCTTTGGTTTGGCTGAATCCTAGAAGCACCATTCTTACCATTCTCACATTTGAAGGTCTGTT AATCCTCATGGAATTGCTAGTGACACATAGGTGTGAAACAGTTGACTATGAAAAGTAGAATAGTTTCCTG TCAACCTAGAGGCTGTTCTTTTATAGAGCAAAGTGTGTGACATTTCATAGTCATAACCTTTTCATTGCTG GTCTTGTCAGCATGTCTTTAGTAGATATATGTAAGTTTCATATTCAATGACACGTTTTGCTTAAAATATG AGTTTTCCCTAGCCACAGAGAGATCCGTCTGGTGCCAGTGCTCCAGCACTGTGGCATGTGTACCACACCT GCTCCATCTTCCTTAGCAGGGAGGCGGACTGCACTTAGCAAACAAACACCAGGGCATACATTGCTGGTCT CCCTTTAATAGACGGTGTTTCAGATAGAGCAGCGAAGGGAGAAAATGCTGAGGCCAAAACAATGCTGAGC ACATATAGCACACACACAGACCGCACACGCACGAACACACACACACATACACACAAACACACACAAAAAT GGGTCGGAACACACATGACTACCGGTTTCCCACTGCTTTTGGAGGGCTGCAACATTCTTCAGCAGTTTGT CTCTTTGTTCTAGTGAACAAGATTTTATCCATGCTTTATATGCCTCTTAGCTGTACAAACAAAGGGTTTA TTTTGTCTTAGTTTTTAACACTGCACTTTTAATGTTTTTGTAACAACTTAAATTTAAAATGCAGCATTTT GGCATTTTCCCAGTAGTGAATTAATCACTTGAAAGCAGATTTTTGTGGCGGAAATAAATGTGTATATATA AAACCTTTTATGTTCACAGCGGCATTAGCCTAGGAATACATTTTATTTGGTTTCTCCAAGTTTCATAGCT AAAGCTAGAATTTGATTTTAAATGCTAACTTATGTCTTAGTTTGTACTGTTTGGAAGAAGCTTATCTAAG TATTACTGATAACAAAGGATTCAACTTCAAGTAAAGGACAAGCATCTTTTAAAATCCACTTAGCATGTTA TGTTTAGGTGCGTATCTGGCGACTGTAGAGATGGGTAGGGATTACAAACTTAGCACTCACTGATGGAAGG TAGGATTTAGGAGGCATTTGCAGTGGAGCAGTCATGAGTACGTTCATCTCTTCAGCCTCACCTCCACAAA TCTGTAGGGTTAGCACTGTTGCATGGCTGGCTGGCCCTTAGCACACGTGCTCGGAAGATCTGCATCTGCA GCTAGACAGCGGGCAGGTCTGCTGACTCGTAGACAGATCTGAGGACAGTTTTCATCTCGATTGAATGTTA CCTCCTTCTCTAGTAAGTTTTTGTGAAAGATTTCAGACTATTAAAATGACTTCTTAAGGAGTAGCTACTA CCTTACCCCCTCCTCTCAGTCTCCATCCAAACCCACTCTCACGGCTACTCCACTAACGCTGTGCTTTGCT TGTAAAGTAACACCATGCAGTTGTGCGCAACCCAAGTATCAAAAGCAGCTACGCGGCTCTGAAAGGTGAT TGTGATAGGTTCTGCTTCTCATCTGCAGATACAATGCAGCAACTCCAAAGTATTAAGATTACAGTAAAGC TGAAGACGCACCTTTGCCCTGGTTAGCAACGCAAAACCTGTGTCGTCGTAAATGCCAAAGTCTTAGTGTG TAAATGTCTGTACACCGTCACAGCGAATGAGCTCACTGCTAATGGCCGAGTTGCTTATTTTCATGAATAA AGATTAGCAAGAAACTTTTAAACCATCTTTTTAAAAAATGTCCTAGTTTGTTATATTTTAGGCATGAAGA ACTAGCTTGGCCTACTGAAGAAATAAAAGATTTAAGAAGAACCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6821844 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6821844 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8173 same embryo
	EMAGE:8177 same embryo
	EMAGE:8174 same embryo
	EMAGE:8176 same embryo
	EMAGE:8175 same embryo
	EurExpress:euxassay_005245 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source