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EMAGE:17623

E430025E21Rik RIKEN cDNA E430025E21 gene ( MGI:2146110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623
"Pseudo-wholemount" of euxassay_005959. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005959_01 euxassay_005959_02 euxassay_005959_03 euxassay_005959_04
EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623
euxassay_005959_05 euxassay_005959_06 euxassay_005959_07 euxassay_005959_08 euxassay_005959_09
EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623
euxassay_005959_10 euxassay_005959_11 euxassay_005959_12 euxassay_005959_13 euxassay_005959_14
EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623
euxassay_005959_15 euxassay_005959_16 euxassay_005959_17 euxassay_005959_18 euxassay_005959_19
EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623 EMAGE:17623
euxassay_005959_20 euxassay_005959_21 euxassay_005959_22 euxassay_005959_23 euxassay_005959_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17623Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17623_wholemount_strong.wlz
17623_wholemount_moderate.wlz
17623_wholemount_weak.wlz
17623_wholemount_possible.wlz
17623_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17623_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 06 22 23
diencephalon lateral wall mantle layer
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18
diencephalon lateral wall marginal layer
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18
telencephalon
strong strong
regionalstrong expression: see section 01 24
cerebral cortex mantle layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 not examined expression: see section 01
cerebral cortex marginal layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 not examined expression: see section 01
telencephalon mantle layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
telencephalon marginal layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
medulla oblongata alar plate mantle layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
medulla oblongata alar plate marginal layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
medulla oblongata basal plate marginal layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
rest of cerebellum mantle layer
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
rest of cerebellum marginal layer
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
pons mantle layer
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
pons marginal layer
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
midbrain
strong strong
regionalstrong expression: see section 04 05 19
midbrain mantle layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
midbrain marginal layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
facial vii ganglion
strong strong
homogeneousstrong expression: see section 04 05 06 07 19 20 21
glossopharyngeal ix ganglion
strong strong
homogeneousstrong expression: see section 05 06 07 17 18 19
trigeminal v ganglion
strong strong
homogeneousstrong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
vagus x ganglion
strong strong
homogeneousstrong expression: see section 07 17
vestibulocochlear viii ganglion
strong strong
homogeneousstrong expression: see section 05 06 07 08 17 18
trigeminal v nerve
strong strong
homogeneousstrong expression: see section 09 17
spinal cord
strong strong
regionalstrong expression: see section 07
spinal cord mantle layer
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15
spinal cord marginal layer
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 08 09 moderate expression: see section 12 13 14 15
cervical ganglion
strong strong
homogeneousstrong expression: see section 07 08 16 17
thoracic ganglion
strong strong
regionalstrong expression: see section 09 moderate expression: see section 10 11
dorsal root ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 12 13 14 15 16 17
neural retina
strong strong
regionalstrong expression: see section 23 24 moderate expression: see section 01 02 03 04
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 10 moderate expression: see section 09 11 12 14 15 16 17 18
nasal septum
moderate moderate
regionalmoderate expression: see section 12 15 16
tongue muscle
moderate moderate
regionalmoderate expression: see section 13 14 15 weak expression: see section 10 11 12 16
stomach
moderate moderate
regionalmoderate expression: see section 01 02 03 04 06 07 08 09 weak expression: see section 05
genital tubercle of male
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13
testis
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 08
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9517
Entity Detected:E430025E21Rik, RIKEN cDNA E430025E21 gene ( MGI:2146110)
Sequence:sense strand is shown

>T9517
CCCGGGTCCCGGCAGCTGACGCTCTGCGGCCCGGGACTCTAGGGGGATCCCTGGGGTGGGAGACGGGGAC
CGGGGCGCCCAGTCCACGCGGCCAGCGTCGGACGGCTGACAGGTTCTTTAATGGAGCGGCCATTTGTCCT
ACAGATCTGGCTTCATGTCATGATCCACCAACCCCTGCTCTGAAGTCAGTCATTCCCACCCTTCAGGTGT
GGACTAGCCAGTCACTCTCACCATGTTGGACTTCCTGGCTGAGAATAATCTCTGTGGCCAGGCCATCCTG
AGGATCGTCTCCTGTGGCAATGCCATCATTGCCGAGGTGCTGCGGCTCTCTGAGTTCATTCCTGCTGTGT
TCCTGCTGAAAGACCGGGCTGACCAGCAGAGATATGGAGACATCATCTTTGACTTCAGCTACTTTAAGGG
TCCAGAGTTCTGGGAAAGCAAACTGGAAGCCAAGCCTGAGCTGCAGGATTTAGATGAAGAATTTCGGGAA
AACAACATAGAAATCGTGACCAGATTTTATTTAGCCTTTCAAAGTGTGCATAAATACATTGTAGACTTAA
ACAGATACCTCGATGACCTCAATGAAGGAGTTTATATTCAGCAAACCTTAGAAACCGTGCTTCTCAGTGA
AGATGGAAAACAACTTCTATGTGAAGCCCTGTACTTATATGGAGTTATGCTGCTTGTCATCGACCAGAAG
ATTGAAGGAGAAGTCAGAGAGAGAATGCTGGTTTCTTACTATCGATACAGTGCTGCTCGGTCCTCTGCTG
ACTCAAACATGGATGATATCTGTAAGCTGCTACGAAGCACAGGATATTCCAGCCAGCCAGGAGCCAAAAG
ACCACCCAACTATCCTGAGAGCTATTTCCAGAGAGTGCCTATCAACGAGACGTTCATCAGCATGGTCATC
GGACGCCTGAGGTCTGATGACATTTACAACCAGGTCTCTGCCTACCCGCTGCCGGAGCACCGCAGCACTG
CCCTGGCCAATCAAGCCGCCATGCTCTACGTGATTCTCTATTTTGAACCTTCCATTCTTCACACCCACCA
AGCAAAAATGAGAGAGATCGTGGATAAGTACTTTCCAGATAACTGGGTAATCAGCATCTATATGGGTATC
ACTGTTAATCTAGCTGATGCCTGGGAACCTTACAAAGCTGCGAAAACTGCTCTCAACAACACCCTTGACC
TCGCCAATGTCAAAGAACAGGCAAGCAGATACGCTAGTGTCAGCGACAGAGTGCGCGCTCAGGTGCAGCA
GTTTCTGAAAGAGGGCTACCTGAGGGAGGAGGTGCTTCTGGACAACATCCCCAGGCTGCTGAACTGCCTG
AGAGACTGCAATGTCGCCATCCGCTGGCTAATGCTCCACACAGCCGACTCAGCCTGTGACCCAAACAACA
AGCGCCTTCGTCAAATCAAGGACCAGATTCTGGCAGACTCTAGGTACAATCCCAAGATCCTCTTCCAGTT
GCTATTGGACACTGCACAATTCGAGTTTATACTCAAAGAGATGTTCAAGCAAATGCTTTCCGAGAAACAG
TCCAAGTGGGAGCATTACAAGAAGGAGGGCTCTGAGCGCATGACCGAGCTTGCTGACGTCTTTTCAGGGG
TGAAACCACTAACCCGAGTGGAGAAAAACGAAAATCTTCAAGCTTGGTTCAGAGAGATCTCAAAACAAAT
ACTGTCTCTAAATTATGATGATTCTACTGCTGCAGGCAGAAAAACTGTGCAACTAATACAAGCTCTGGAA
GAGGTTCAGGAGTTTCACCAGTTGGAATCAAATCTACAAGTTTGCCAGTTTCTAGCTGACACCCGAAAGT
TTCTCCATCAAATGATCAGAACCATCAACATCAAGGAGGAAGTCCTGATCACAGTGCAGATCATCGGGGA
CCTTTCCTTTGCCTGGCAGTTAATTGACAGTTTCACATCCATCATGCAAGAAAGCATAAGGGTAAACCCA
TCCATGGTTACCAAACTCAGAGCCACGTTCCTAAAGCTTGCCTCTGCCCTTGACCTTCCCCTGCTACGTA
TTAATCAAGCAAATAGCCCTGATCTCCTCAGTGTGTCTCAGTATTATTCAGGAGAATTGGTGTCATACGT
GAGAAAGGTTTTGCAGATCATTCCAGAAAGCATGTTCACATCTCTCCTGAAGATCATAAAGCTTCAGACC
CATGACATCATGGAAGTGCCTACACGCCTGGACAAGGACAAGCTGCGGGACTACGCTCAGCTTGGCCCGA
GATATGAGGTTGCCAAGCTCACTCACGCCATCTCCATCTTTACTGAGGGCATCTTGATGATGAAAACGAC
CCTGGTGGGCATCATCAAGGTGGATCCCAAGCAGTTGCTGGAAGACGGAATAAGGAAAGAGCTGGTTAAA
CGCGTGGCTTTCGCCCTTCACCGGGGACTGATATTCAACCCTCGTGCCAAGCCAAGTGAGTTGATGCCCA
AACTGAAAGAGCTGGGGGCCACCATGGATGGGTTCCATCGCTCCTTTGAGTATATACAGGACTACGTCAG
CATTTACGGTCTGAAGATTTGGCAGGAGGAAGTCTCTCGTATTATAAATTATAACGTGGAACAGGAGTGT
AATAACTTCTTAAGAACAAAGATTCAAGACTGGCAGAGTATGTACCAATCCACTCATATACCAATCCCGA
AGTTTGCACCTGTGGATGAGTCTATAACATTTATTGGTCGACTCTGCAGAGAAATCTTGCGGATCACAGA
CCCAAAAATGACATGTTACATAGACCAGCTGAACACTTGGTATGACGTGAAAACTCATCAAGAAGTGACC
AGCAGTCGCCTCTTCTCAGAAATCCAGACCACCTTGGGGACTTTTGGTCTGAATGGATTGGACAGGCTTC
TGTGCTTCATGATTGTGAAAGAGCTACAGAATTTCCTCAGTATGTTTCAGAAAATTATCCTGAAAGAGAG
AACTGTTCAAGAAACTTTAAAGATGCTCATGAGTGCTGTTAACCCTCTAAAGAGCATAGTGGCAAATTCA
AGTAAAGTTTATCTTTCTGCCATCACCAAAACACAGAAGATCTGGAGTGCTTACCTGGAGGCTATAATGA
AGGTTGGACAGATGCAGATCCTGAGACAACAGATCGCCAATGAGCTAAACTCTTCCTGTCGATTTGATTC
CAGACATCTGGCAGCTGCCCTGGATAATCTCAATAAGGCTCTCCTGGCAGACATCGAAGCCCACTACCGG
GACCCTTCGCTTCCTTACCCCAAAGAAGATAACACACTCTTATATGAAATCACAGCCTATCTGGAGGCGG
CTGGCATTCACAACCCCCTGAATAAGATTTACATAACAACGAAGCGCTTACCCTATTTCCCGATTGTCAA
CTTTCTGTTTTTGATCGCGCAGTTGCCAAAACTTCAGTATAACAAAAATCTAGGCATGGTCTGCCGGAAG
CCTGCCGACCCTGTTGACTGGCCACCGCTCGTCCTAGGTCTGCTCACGCTGCTGAAGCAGTTCCACTCCC
GGTACACCGAGCAATTCCTGGCACTGATTGGCCAGTTTATTCGCTCCACAATGGAGCAGTGCACAAGCCA
GAAGATGCCTGAAATGCCTGCAGATGCTGTGGGCGCCCTTCTCTTCCTGGAGGATTATGTTCGGTATACA
AAACTCCCCCGAAGGGTGGCTGAAGCCCATGTGCCTAACTTCATTTTTGATGAATTCCGAACAGTCCTGT
GACTTCTTTCTCCTGCTCCAGTGACAGGATGTTCTATGACTAGAATGAACTTGGACGTGAGAAAGATACT
TGACTCCTCATGCAACGTGCATTTTCCTCCTCTGTGGGAACCAGCCATTCTAAGGAAAGTGGCTCTCAAC
TGTCTAAATCATGGCATCGTAAGGAATTTTTATCATGTAGATGCAGAACTGTTTTGTACTTGCCTCTTTT
AAATGCTCAGTATAACTGATGGATGAAGCCATTCCATCTTATGTTCTAAGGATCTGTTTGTGCAACTCCA
GACCTTCAGTAAAACAGCATTGCTAGTAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30532658 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30532658 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17624 same embryo
 EMAGE:17625 same embryo
 EMAGE:17626 same embryo
 EMAGE:17622 same embryo
 EurExpress:euxassay_005959 same experiment
 MGI:4824455 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS