EMAGE mouse gene expression spatial database

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EMAGE:7289

Rtn4 reticulon 4 ( MGI:1915835)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004344. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004344_01	euxassay_004344_02	euxassay_004344_03	euxassay_004344_04

euxassay_004344_05	euxassay_004344_06	euxassay_004344_07	euxassay_004344_08	euxassay_004344_09

euxassay_004344_10	euxassay_004344_11	euxassay_004344_12	euxassay_004344_13	euxassay_004344_14

euxassay_004344_15	euxassay_004344_16	euxassay_004344_17	euxassay_004344_18	euxassay_004344_19

euxassay_004344_20	euxassay_004344_21	euxassay_004344_22	euxassay_004344_23	euxassay_004344_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7289_wholemount_strong.wlz

7289_wholemount_moderate.wlz

7289_wholemount_weak.wlz

7289_wholemount_possible.wlz

7289_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7289_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

shoulder mesenchyme

moderate

regional

moderate expression: see section 23

upper arm mesenchyme

moderate

regional

moderate expression: see section 23 24

foot mesenchyme

moderate

regional

moderate expression: see section 06 07 19 20

lower leg mesenchyme

moderate

regional

moderate expression: see section 03 04 05 22 23 24

upper leg mesenchyme

moderate

regional

moderate expression: see section 03 04 05 22 23

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 17 18 19

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 14 15 16 17 18 19

not examined

regional

not examined expression: see section 01 02 03 22 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 22 23 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5087
Entity Detected:	Rtn4, reticulon 4 ( MGI:1915835)
Sequence:	sense strand is shown >T5087 CCACGCGTCCGCCAAAACGTCAAATCCTTTCCTTGTAGCAATACATGATTCTGAGGCAGATTATGTCACA ACAGATAATTTATCAAAGGTGACTGAGGCAGTAGTGGCAACCATGCCTGAAGGTCTAACGCCAGATTTAG TTCAGGAAGCATGTGAAAGTGAACTGAACGAAGCCACAGGTACAAAGATTGCTTATGAAACAAAAGTGGA CTTGGTCCAGACATCAGAAGCTATACAAGAGTCAATTTACCCCACAGCACAGCTTTGCCCCTCATTTGAG GAAGCTGAAGCAACTCCGTCACCAGTTTTGCCTGATATTGTTATGGAAGCGCCATTAAATTCTCTCCTTC CAAGCACTGGTGCTTCTGTAGCGCAGCCCAGTGCATCCCCACTAGAAGTACCGTCTCCAGTTAGTTATGA CGGTATAAAGCTTGAGCCTGAAAATCCCCCACCATATGAAGAAGCCATGAGTGTAGCACTAAAAACATCG GACGCAAAGGAAGAAATTAAAGAGCCTGAAAGTTTTAATGCAGCTGCTCAGGAAGCAGAAGCTCCTTATA TATCCATTGCATGTGATTTAATTAAAGAAACAAAGCTCTCCACTGAGCCAAGTCCAGAGTTCTCTAATTA TTCAGAAATAGCAAAATTTGAGAAGTCGGTGCCTGATCACTGTGAGCTCGTGGATGATTCCTCACCCGAA TCTGAACCAGTTGACTTATTTAGTGATGATTCAATTCCTGAAGTCCCACAAACACAAGAGGAGGCTGTGA TGCTAATGAAGGAGAGTCTCACTGAAGTGTCTGAGACAGTAACACAACACAAACATAAGGAGAGACTTAG TGCTTCACCTCAGGAGGTAGGAAAGCCATATTTAGAGTCTTTTCAGCCCAATTTACATATTACAAAAGAT GCTGCATCTAATGAAATTCCAACATTGACCAAAAAGGAGACAATTTCTTTGCAAATGGAAGAGTTTAATA CTGCAATTTATTCCAATGATGACTTACTTTCTTCTAAGGAAGACAAAATGAAAGAAAGTGAAACATTTTC CGATTCATCTCCCATTGAGATAATAGATGAGTTTCCCACATTTGTCAGTGCTAAAGATGATTCTCCTAAG GAGTACACTGACCTAGAAGTATCCAACAAAAGTGAAATTGCTAATGTCCAGAGCGGGGCCAATTCGTTGC CTTGCTCAGAATTGCCCTGTGACCTTTCTTTCAAGAATACATATCCTAAAGATGAAGCACATGTCTCAGA TGAATTCTCCAAAAGTAGGTCCAGTGTATCTAAGGTGCCCTTATTGCTTCCAAATGTTTCTGCTTTGGAA TCTCAAATAGAAATGGGCAACATAGTTAAACCCAAAGTACTTACGAAAGAAGCAGAGGAAAAACTTCCTT CTGATACAGAGAAAGAGGACAGATCCCTGACAGCTGTATTGTCAGCAGAGCTGAATAAAACTTCAGTTGT TGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCTTATTCCTGCTGCTG TCTCTGACAGTGTTCAGCATTGTCAGTGTAACGGCCTACATTGCCTTGGCCCTGCTCTCTGTGACTATCA GCTTTAGGATATATAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATA TTTGGAATCTGAAGTTGCCATATCAGAGGAATTGGTTCAGAAATATAGTAATTCTGCTCTTGGTCATGTG AACAGCACAATAAAAGAATTGAGGCGTCTCTTCTTAGTTGATGATTTAGTTGATTCCCTGAAGTTTGCAG TGTTGATGTGGGTATTTACTTACGTTGGTGCCTTGTTCAATGGTTTGACACTACTGATTTTAGCTCTGAT CTCACTCTTCAGTATTCCTGTTATATATGAACGGCATCAGGCGCAGATAGATCATTATCTAGGACTTGCA AACAAGAGCGTTAAGGATGCCATGGCCAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCAGAAT GAAAAGGCCCCAAACAGTAGACATTCATCTTTAAAGGGGACACTCCCTTGGTTACGGGGTGGGCGGGTCA GGGGTGAGCCCTGGGTGGCCGTGCAGTTTCAGTTCTTTATTTTTAGCAGTGCACTGTTTGAGGAAAAATT ACCTGTCTTGACTTCCTGTGTTTATCATCTTAAGTATTGTAAGCTGCTGTGTATGGATCTCATTGTAGTC ATACTTGTTTTCCCCAGATGAGGCACTTGGTGAATAAAGGATGCTGGGAAAACTGTGTGTTATATTCTGT TGCAGGTAGTCTGGCTGTATTTGGAAAGTTGCAAAGAAGGTAGATTTGGGGGCAGGAAAAACAACCCTTT TCACAGTGTACTGTGTTTGGTTGGTGTAAAACTGATGCAGATTTTTCTGAAATGAGATGTTTAGATGAGC ATACTACTAAAGCAGAGTGGAAAAATCTGTCTTTATGGTATGTTCTAGGTGTATTGTGATTTACTGTTAG ATTGCCAATATAAGTAAATATAGACATAATCTATATATAGTGTTTCACAAAGCTTAGATCTTTAACCTTG CAGCTGCCCCACAGTGCTTGACCTCTGAGTCATTGGTTATACAGTGTAGTCCCAAGCACATAAACTAGGA AGAGAATGTATTTGTAGGAGCGCTACCACCTGTTTTCAAGAAAACATAGAACTCCAACGTAACCGTCATT TCAAAGATTTACTGTATGTATAGTTGATTTTGTCACAGACTCTGATTTTGTGGACTGAATTTAATGCTTC CAAATGTTTGCAGTTACCAAACATTGTTATGCAAGAAATCATAAAATGAAGACTTATACCATTGTGTTTA AGCTGTATTGAATTATCTGTGGAATGCATTGTGAACTGTAAAGCAAAGTATCAATAAAGCTTATAGACTT AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5323152 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5323152 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7288 same embryo
	EMAGE:7290 same embryo
	EMAGE:7285 same embryo
	EMAGE:7287 same embryo
	EMAGE:7286 same embryo
	EurExpress:euxassay_004344 same experiment
	MGI:4827830 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source