EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7424

Eps8 epidermal growth factor receptor pathway substrate 8 ( MGI:104684)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004434. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004434_01	euxassay_004434_02	euxassay_004434_03	euxassay_004434_04

euxassay_004434_05	euxassay_004434_06	euxassay_004434_07	euxassay_004434_08	euxassay_004434_09

euxassay_004434_10	euxassay_004434_11	euxassay_004434_12	euxassay_004434_13	euxassay_004434_14

euxassay_004434_15	euxassay_004434_16	euxassay_004434_17	euxassay_004434_18	euxassay_004434_19

euxassay_004434_20	euxassay_004434_21	euxassay_004434_22	euxassay_004434_23	euxassay_004434_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7424_wholemount_strong.wlz

7424_wholemount_moderate.wlz

7424_wholemount_weak.wlz

7424_wholemount_possible.wlz

7424_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7424_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

adrenal gland

moderate

regional

moderate expression: see section 09 10 17 18

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15 16

pancreas

moderate

regional

moderate expression: see section 08 09 10 11 12 13

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 09 10

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22

nasal septum

moderate

regional

moderate expression: see section 11 12 13 14 15

viscerocranium

moderate

regional

Expression in the turbinate bone.

rectum

moderate

regional

moderate expression: see section 12 13

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

metanephros

moderate

spotted

moderate expression: see section 08 09 10 17 18 19

testis

moderate

regional

moderate expression: see section 06 07 08 19 20 21

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7552
Entity Detected:	Eps8, epidermal growth factor receptor pathway substrate 8 ( MGI:104684)
Sequence:	sense strand is shown >T7552 GACCCACGCGTCCGGAGTTCTCCATCAATGCTTCCCATTGTTATTTGCTGCTATGTTAGTCTCCCCACCT CGGGGAAGGGCTTTACCTCCTCAGCGGTGGTGATTTCCTTGTTTAAAAGAGGAGGAAGTCTTAGCTGAGG CCTGGGAGAGTCCAAAGGAGAGACTTTGATGAGTATAGCGGAACACCGAAACTGAAGCAGTCCCACCCTC TCGGTGTATGAACCAAAGGTCACTGGACACATCCGTGAGCAGGGAGCGATGCTCTCTCAGTCTTGCCTTG ACAGTTGCTTGGACGAGTTTTACCTCAGCAGTGCAGGAAGAACAGAGGCGTGAGAGCCGGCATGAATGGT CATATGTCTAACCGCTCCAGTGGGTATGGAGTCTACCCTTCTCAACTGAATGGTTACGGATCTTCACCAC CCTATTCCCAGATGGACAGAGAACACAGCTCAAGAACAAGTGCAAAGGCCCTTTATGAACAAAGGAAGAA CTATGCCCGAGACAGTGTCAGCAGTGTGTCGGACGTGTCCCAGTACCGCGTGGAACACTTGACCACCTTC GTGCTGGATCGGAAAGATGCAATGATCACTGTCGAGGACGGAATAAGAAAGCTGAAGTTGCTGGATGCCA AGGGCAAAGTGTGGACTCAAGATATGATTCTCCAAGTGGATGACCGAGCTGTGAGCCTGATTGACTTAGA GTCAAAGAATGAATTGGAGAATTTTCCTCTAAACACAATCTCGCATTGTCAAGCAGTGGTGCATGCATGC AGCTATGACTCCATTCTCGCCTTGGTATGCAAAGAGCCAACGCAGAGCAAGCCAGACCTTCACCTTTTCC AGTGTGATGAGGTTAAGGCAAACCTAATTAGTGAAGATATCGAAAGTGCAATCAGTGACAGTAAAGGTGG GAAACAGAAGAGGCGGCCGGAGGCCCTGAGGATGATTGCCAAAGCAGATCCTGGCATCCCTCCTCCTCCC AGAGCTCCTGCCCCTGTGCCACCGGGGACTGTCACACAGGTGGACGTTAGGAGTCGCGTAGCAGCCTGGT CTGCCTGGGCAGCTGACCAGGGTGACTTCGAGAAGCCCCGGCAGTACCACGAGCAAGAAGAGACGCCCGA GATGATGGCAGCCCGGATCGACAGGGATGTGCAAATCTTAAACCATATTTTGGATGACATTGAATTTTTT ATCACCAAACTCCAAAAAGCCGCCGAAGCGTTTTCTGAGCTTTCTAAAAGGAAGAAAAGTAAGAAAAGTA AAAGGAAAGGACCTGGAGAGGGCGTTTTAACACTGAGGGCAAAACCGCCACCTCCTGACGAGTTTGTTGA CTGTTTCCAGAAGTTTAAACATGGATTCAACCTTCTGGCCAAGTTGAAGTCCCATATCCAGAACCCGAGT GCTTCAGATCTGGTTCATTTTTTGTTTACTCCACTAAATATGGTGGTCCAGGCAACAGGTGGCCCTGAAC TGGCCAGTTCGGTACTCAGCCCACTGTTGACAAAAGACACAGTTGATTTCTTAAACTACACAGCCACTGC GGAGGAACGGAAGCTGTGGATGTCACTGGGAGATAGTTGGGTGAAAGTGAGAGCAGAGTGGCCGAAAGAA CAGTTCATCCCACCTTACGTCCCGAGGTTCCGCAACGGCTGGGAGCCCCCGATGCTGAACTTCATGGGCG CGCCCACAGAGCAAGACATGTATCAACTGGCCGAGTCCGTGGCCAACGCAGAACACCAGCGCAAACAGGA CAGCAAGAGGCTGTCCACAGAGCATTCCAATGTGTCCGACTATCCTCCAGCCGACGGATATGCGTACAGT AGCAGCATGTACCACAGAGGACCACATGCAGACCACGGGGAGGCTGCCATGCCTTTCAAGTCAACTCCTA ATCACCAAGTAGATAGGAATTATGACGCAGTCAAAACACAACCCAAGAAATACGCCAAATCCAAGTACGA CTTTGTGGCGAGGAACAGCAGCGAGCTCTCGGTTATGAAAGATGATGTCTTAGAGATACTCGACGATCGA AGGCAGTGGTGGAAAGTCCGGAATGCCAGTGGAGACTCTGGGTTTGTGCCAAATAACATTCTGGATATCA TGAGAACTCCAGAATCTGGAGTGGGGCGCGCTGACCCCCCATACACACATACCATACAGAAACAAAGGAC GGAATACGGCCTGAGATCAGCTGACACTCCTTCTGCCCCATCACCCCCTCCAACGCCAGCACCCGTTCCG GTCCCCCTTCCACCTTCTGTACCAGCACCCGTTTCTGTGCCCAAGGTTCCAGCAGATGTCACCCGCCAGA ACAGCAGCTCCAGTGACAGTGGGGGCAGCATTGTGCGGGACAGCCAGAGATACAAACAACTCCCAGTGGA CCGAAGGAAGTCCCAGATGGAAGAGGTTCAGGATGAGCTCTTCCAGAGGCTGACCATCGGGCGCAGTGCT GCGCAGAGGAAGTTCCACGTGCCACGGCAGAACGTTCCAGTGATCAATATCACTTATGACTCCTCACCGG AAGAAGTAAAGACTTGGCTGCAGTCAAAGGGATTCAATCCCGTGACTGTCAATAGCCTCGGGGTGTTGAA CGGAGCACAACTCTTTTCTCTCAACAAAGACGAACTGAGGTCTGTCTGCCCGGAAGGTGCCAGAGTCTTT AACCAAATCACTGTTCAGAAAGCTGCTTTGGAGGACAGTAATGGAAGCTCCGAGTTACAAGAGATCATGC GGAGACGGCAGGAGAAGATCAGCGCCGCTGCGAGCGACTCGGGAGTGGAGTCTTTTGATGAAGGGAGCAG CCACTGAGTCCATGAACTTCCTTATTCTTGGTGTGGTCGTTGAACAGTGATGGACATGCTTTGTTTTAAG AAGCCTTGAAGGGAATGTCAAAGCTGTCGTCTTGGTATATGTAATTTATCGCCATATAAGGAAACAGTAT ATGCCTGAGTAAGCAGAGGACCCGCTGCTTCTGTGCACATTAGTTTGATTAAAACTGAGAAGCGGGTAGG TGAGATGGCTCAGCAAGTAAAGGTGCTTGCTGCCAAGCCCAATGACCCAAGTTCGAGTCCCTGGGTCTAC ATGGTAGGAGAGAGCTGGCTTCTGCAAGTTGTCCTCTGACCACCACACATAAATAAATAACAAATGTAAT TTACAAACTTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240899 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240899 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7425 same embryo
	EMAGE:7429 same embryo
	EMAGE:7426 same embryo
	EMAGE:7428 same embryo
	EMAGE:7427 same embryo
	EurExpress:euxassay_004434 same experiment
	MGI:4824602 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source