EMAGE mouse gene expression spatial database

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EMAGE:7566

Hs3st3a1 heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 ( MGI:1333861)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004694. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004694_01	euxassay_004694_02	euxassay_004694_03	euxassay_004694_04

euxassay_004694_05	euxassay_004694_06	euxassay_004694_07	euxassay_004694_08	euxassay_004694_09

euxassay_004694_10	euxassay_004694_11	euxassay_004694_12	euxassay_004694_13	euxassay_004694_14

euxassay_004694_15	euxassay_004694_16	euxassay_004694_17	euxassay_004694_18	euxassay_004694_19

euxassay_004694_20	euxassay_004694_21	euxassay_004694_22	euxassay_004694_23	euxassay_004694_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7566_wholemount_strong.wlz

7566_wholemount_moderate.wlz

7566_wholemount_weak.wlz

7566_wholemount_possible.wlz

7566_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7566_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thoracic intervertebral disc

moderate

regional

moderate expression: see section 11

humerus

moderate

regional

moderate expression: see section 05 06 22

hand mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 06 07 20 21 22 23 24

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 07 24

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 08 09 10 24

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 09 10 24

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 09 24

hindlimb digit 5 metatarsal

moderate

regional

moderate expression: see section 09 23 24

foot mesenchyme

moderate

regional

moderate expression: see section 05 06 07 22 24

femur

strong

regional

strong expression: see section 17 moderate expression: see section 08

pectoral girdle and thoracic body wall muscle

moderate

regional

moderate expression: see section 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 15 16 weak expression: see section 17

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10 11 15 16 17 weak expression: see section 09

pons ventricular layer

moderate

regional

moderate expression: see section 08 18 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 weak expression: see section 13

spinal cord ventricular layer

moderate

regional

moderate expression: see section 12 13 14

nasal septum

weak

regional

weak expression: see section 12 13 14 15 16

viscerocranium

weak

regional

Expression in the turbinate bone.

mandible

moderate

regional

moderate expression: see section 03 04 05 06 23 24 weak expression: see section 07 08 11 12 20 21 22

maxilla

moderate

regional

moderate expression: see section 03 04 23 24

renal cortex

strong

regional

strong expression: see section 06 07 08 17 18 19 20 moderate expression: see section 09 10 16

trachea

strong

regional

strong expression: see section 14 15

clavicle

moderate

regional

moderate expression: see section 11 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8660
Entity Detected:	Hs3st3a1, heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 ( MGI:1333861)
Sequence:	sense strand is shown >T8660 GCGTCCCACGTGTGGCGGTAGCGGCTGAGGAGGCTGCGGGGAGAGCCCAAGAGCACAGTGGCAGCGGTGG CTGACAGGTCCCGCACAGCGGGGCCCCTCCAAGCTGTTCCCAGCTGGGGCCCCAGAGGAGTGGGTAGACT TCCGGAGCGCTCAAGAGGCCAGAAGGCTTGAGTCCCATTCGGATAGAGGGACTAGGGGGACTTTGCTTGC TCGGTTGCTGAGACGAGCTGAGCCAGGACTCAGGGCACGCTCCTGGGCGGGCCAGGAGAGCAGAAGCTGG AAAAAGGGTCACTCGTTCTAACCGGAGCGTACACAATTCTTCGGCCCGAGAGTCCTGCGCCCGCTCCACG GGGGACTGAGCCAAGGATGTGTTATCGCTGAGCCAAGTTCCGCACCGCTCAGGACCCTGCTGAGCCGAGC ACCTTCGCCACTGCCGCCGCAGAGACTCCCTTGCCGGGCGACTCTCGGAGACCCAGGGCGCGGAGCTCTG CGCAGCTAGGCTCAGGACTCGGGCGCGTTTCAGACCCAAGACCATGGTTTCTTAAGCACGGCCCCGCACC CCTTTCCTCCTCGTCGCCCGAAGCCCAGGACCCCAAGAAGGGCTCGCAGTGCTTGGTCCCTAGTTCCAGC CCCTGCTGCCGCCCAGCGGGGCAGCTCTCAGGCACGCAGGGCGGCTCAGGCGATGCGGCCGCGGGGCAGC ACGCTGGCTGGCAGCGGGGATGGCGGAGCTCCCAGCACAGTCGTGGCCTAGCATGGCCCGCTCGCCGTCC GAGGTCGCCTCTGGCTAGGATGGCCCCCTCGGGTCCCACCGGCGCCCAGCCCAGCCCGGCGGAGCCACTG TCGCGCAGCATCTTCAGGAAGTTCTTGCTGATGCTCTGCTCGCTACTCACTTCCCTCTACGTCTTCTACT GCCTGGCGGAGCGCTGCCCACCCGGCTCGGGCCCCGTCGCGGGGGTCCCGGGGCGCGGCGTCCCTGCAGG ACCCAGGGAACTGGCGATGTGGCCCGCGGGAGCGCCAAGGAAGCGCCTCCTGCAGCTGAGGCAACGGAGG AGGCGCGGGCGGTCGGGGCCAGGAGACAGCAGCGACCAGGAGGAGCAGAGCCCTGGGCTGGCCGCCGCAC CAGGAGGCTCCGGGGCGGGAAGCAGCGTGGCCGAGGCCCAGCCGGGGACCCTGGCCTTACTTCTGGACGA GGGCAGCAAGCAGCTGCCGCAGGCCATCATCATCGGCGTGAAGAAGGGCGGCACGCGGGCGCTGCTGGAG TTCCTGCGCGTGCACCCCGACGTGCGTGCCGTGGGTGCTGAGCCCCACTTTTTTGACCGCAGCTACCACA AGGGCCTCGCCTGGTACCGGGATTTGATGCCCAGAACCCTCGAGGGACAGATCACCATGGAGAAGACGCC CAGCTACTTTGTGACCCGGGAGGCACCCGCGCGCATCTCGGCCATGTCCAAGGACACCAAACTCATCGTG GTGGTGCGCGACCCGGTGACCAGGGCCATCTCGGACTACACGCAGACGCTGTCCAAGCGGCCGGACATCC CCAGCTTCGAGAGCCTGACGTTCCGCAACCGCAGCGCGGGCCTCATAGACACGTCCTGGAGCGCCATCCA GATCGGCCTGTACGCCAAGCACCTGGAGCCCTGGCTGCGCCACTTCCCGCTCGGCCAGATGCTCTTCGTG AGCGGGGAGCGGCTGGTCAGCGACCCGGCCGGCGAGCTGCGCCGCGTGCAGGATTTCCTGGGCCTCAAGC GCATCATCACCGACAAGCACTTCTACTTCAACCAGACCAAGGGCTTCCCGTGCCTCAAGAAGGCGGAGGG CAGCGGCAAGCCGCACTGCCTGGGCAAGACTAAGGGCCGCGCTCACCCCACCATTGCGCGGGAGGTGCTG CGGCAGCTGCGTGACTTTTACCGGCCCTTCAACCGCAAGTTCTACCAGATGACCGGCCGCGACTTTGGCT GGGATGGATAGCAGAAGTAATTTAAAAAGAAAAAAAAATAATATCAAAATATAATATATTTTTTTACCAG TCGGTAGAGAAAAGACGGTTTAATATTTCTGTATTAAGAGTATGTGTTGCAGTATTTTTTTTTCTTCCGG TCCCCGTGGATAACCAGCACCAAACATGCGCATAGCAGAAAAGGAGATTTCATGTATCTAAGCATCTCAT ATCTCCCGTTCCTTTGCGTTATAAGCTTTCATTCACAAAGAATGAGCACCAGTACTCCACTAAACTGTTT TAGGAAACCCCCCAGGGTAAACCTCTCTTGTTACCCACAAAGACCTGATAGAACTGAGACTTGGCCTTCC AGTTTTCTTTCTCCTGTGCCACTTTTCTCCTAAGTCATTTCTCGCTAAGATACTCAGCATGCACAGGGCC ATTTTCGATGGTAGTTTTGGCCCTTACACCACCACTGCAAGCATGTAGTGTGTTTCTCGAGATCATCATT TTCTAGTTCAGAGATGAGGGAAACTGAGTCTCAGGGAGGTCGAATGACTCATCTTAAAGCTGGATAGTGG ATCCTGGACAAAAATCCTGTCTGCCGCTGCTTTTTCTCTATTTTATGACACCGCCCCTGACCCCACCCGG CTAGAGCATATAAGACACCTGGAGCTTTAGAAGCTCCGGAGTGGTCCGGAAAGCACAAGGGGGACTTGTC ACTGCAATGTGAGTCCCGGGCTGGCCTATGGCACACAGCCTCATTACAAAGCTGTATTCAAGGAAGTCAC AGAGATGGGGACACAAGCCCTCTAGGGAGCCTATCTTGCCCCTTGGCTGATCAAAGGATGCTTAGACAAG GAGATCTGACCGATGGGTGGTCATCTGAGTCCCAGAGAAATTACTGAGCTCTTCTGTTGAGAGAAGATGG ACAGACAGAACCAGGAGTGTCGCAGAGTAGCACTTACTAGCTGTTGGTTTGACTTCTTTATTTCTTTTTA AAGAAAAACCCAATTCAATTAGAACTTATCAATACGAATTCCATTTTCCTTATTTTAGAACCCCCCCCCT TTTCTTTGTAGTATCTCCGGTCTGACCTCAGTTAACAACTTCTTCCCAGCTCCCCCTACAGATTGAAGCT GTCACTCGGTCACTTTATCCATGTCCATCATTTCCCTCTCATATGAATTATAACCTAATGCTCATTAATG TTTGGAAAATATTCTAAGCAAAGAGACATAGTTGTGGATGGAATGGTGATGTCTCCTCCCTTCCCTGTCT AAATGGCTCAAATGAATCAATAGTTTATTAAGAGTACTCCTTGCTCTCTGAGTGGGTATTAACGAGATTA ACATTAATGGGAATTAAATTGGTTTAGCGAGGAGCACGTATGTGCTGATCATATAAAACATTCATAATTA CTCTGTCAGCGCCATTTTACCACAGGTTTAAGACCATGAGGAACATGTTTGCTATCCCATCAATATTTCT CTTATCTGCTGAAATTTAAAGGGCCAGACACGCTAATACAGTAAAAGAGTGTGCTGTTTTCTCTGCTGTC AATCAAGAGAAACTAGAGTTTGTTTTACAATATGCTGGGTGACTGTGGGTTTGTCATAGGTGCCCTGTCA GGGATGTACATGCCTCTTAGGAAGACTAATACGGCGTTAAAATGAAGTGAAGACATCATTTTAAGTTTCT AAATATTTTGTCATCTTTGGATTTATTTGCTGATATCTCATGCATGGGAATTAAGCCCTGAACTTAGGTC GCCCTTTCCACTTTGTCTCCGACATTTCTATGCCCTTGGTGTCCAGAGCCAGTCTGTTTTTAGTCGCTGA GAGCCATCACCTGTGGCCACCGGAGACTCACTTTCAGCCCTGCTCATCTTTTGATTTGTTTGTGTTTCTG AATCTTTTGTATTCCCCTCTCTGCCTGCCATTACGCAAAAGCAGCTTGCACAAAACCCAGAATTGTATCT AACAGCTGCACTCAGCAACCTCCCTCTTTTCCCATGGAAGAGATAATAAAAGAACAAAATTGAATGCAAA GGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400013 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400013 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7570 same embryo
	EMAGE:7571 same embryo
	EMAGE:7567 same embryo
	EMAGE:7569 same embryo
	EMAGE:7568 same embryo
	EurExpress:euxassay_004694 same experiment
	MGI:4825454 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source