EMAGE mouse gene expression spatial database

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EMAGE:31731

Col5a2 ( MGI:88458)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009230_01	euxassay_009230_02	euxassay_009230_03	euxassay_009230_04	euxassay_009230_05

euxassay_009230_06	euxassay_009230_07	euxassay_009230_08	euxassay_009230_09	euxassay_009230_10

euxassay_009230_11	euxassay_009230_12	euxassay_009230_13	euxassay_009230_14	euxassay_009230_15

euxassay_009230_16	euxassay_009230_17	euxassay_009230_18	euxassay_009230_19	euxassay_009230_20

euxassay_009230_21	euxassay_009230_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

regional

moderate expression: see section 10 13 weak expression: see section 07 08 09 11 14 15 16

midbrain ventricular layer

moderate

regional

moderate expression: see section 10 11 weak expression: see section 06 07 08 09 12 13 14 15

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10 weak expression: see section 09 13

pons ventricular layer

moderate

regional

moderate expression: see section 10 weak expression: see section 05 06 07 08 09 11 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2545
Entity Detected:	Col5a2, ( MGI:88458)
Sequence:	sense strand is shown >T2545 TGGTTCCAAGAAACACCCAGCCCGCACTTGTGATGATTTAAAGCTTTGCCATCCCACAAAGCAGAGCGGT GAATATTGGATTGATCCTAACCAGGGATCAGCTGAAGATGCAATCAAAGTTTACTGCAATATGGAAACAG GAGAGACATGTATTTCAGCAAACCCAGCCAGTGTTCCACGTAAAACCTGGTGGGCCAGCAAATCCCCTGA CAATAAGCCTGTGTGGTACGGTCTTGACATGAATAGAGGATCTCAGTTCACATATGGAGATTACCAGTCT CCTAACACAGCTATCACTCAGATGACCTTTTTGCGTCTTTTATCAAAAGAAGCCTCGCAGAACCTTACTT ACATCTGTAGGAACACTGTTGGATATATGGATGATCAAGCTAAAAACCTCAAGAAAGCTGTGGTCCTTAA AGGGTCAAACGACTTAGAAATAAAAGGAGAAGGAAACATCAGATTCAGATACACAGTTCTTCAAGACACC TGCTCTAAGCGAAATGGAAATGTAGGCAAAAC
Notes:	The probe template was PCR amplified from IMAGE:1382148 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1382148 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009230 same experiment
	EMAGE:29739 same embryo
	EMAGE:31729 same embryo
	EMAGE:30960 same embryo
	EMAGE:30963 same embryo
	EMAGE:31446 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source