EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30662

Akap10 ( MGI:1890218)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004945_01	euxassay_004945_02	euxassay_004945_03	euxassay_004945_04	euxassay_004945_05

euxassay_004945_06	euxassay_004945_07	euxassay_004945_08	euxassay_004945_09	euxassay_004945_10

euxassay_004945_11	euxassay_004945_12	euxassay_004945_13	euxassay_004945_14	euxassay_004945_15

euxassay_004945_16	euxassay_004945_17	euxassay_004945_18	euxassay_004945_19	euxassay_004945_20

euxassay_004945_21	euxassay_004945_22	euxassay_004945_23	euxassay_004945_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7995
Entity Detected:	Akap10, ( MGI:1890218)
Sequence:	sense strand is shown >T7995 GACAAGAGGTAATCCAAACAGAGTTCACTGGCTGAGCCTGTCTCTCCATCTAAAAGACATGAGACTCCAG CATCTTCTGTAACTGAGGCTCTTGACAGGAGGTTGGGGGATTCTAGCTCAGCCCCACTGCTTGTGACTCA ATCAGAAGGAACTGACCTGAGTAGTAGAACTCAGAACCCTCAGAACCACTTGCTGCTTTCCCAGGAAGGC CACAGTGCCCGTTCTCTCCATCGTGAGGTGGCCAGAACAGGAAGTCATCAGATTCCCACCGACTCCCAGG ACTCCTCCTCCAGACTTGCAGTAGGCAGTAGAAACAGCTGCTCTTCTCCACTGAGAGAACTGTCAGAAAA ACTAATGAAAAGTATAGAACAAGATGCAGTGAATACTTTTACCAAATATATATCTCCAGATGCTGCTAAG CCAATACCAATTACAGAAGCCATGAGAAACGACATCATCGCAAAGATTTGTGGAGAAGATGGACAGGTGG ATCCCAACTGTTTCGTTCTGGCACAGGCTGTAGTCTTTAGTGCAATGGAGCAAGAGCACTTTAGTGAGTT TCTGCGAAGTCACCATTTCTGTAAATACCAGATTGAAGTGCTGACCAGTGGGACTGTTTACCTGGCTGAT ATCCTCTTCTGTGAGTCAGCCCTCTTTTATTTTTCTGAGTACATGGAAAAAGAAGATGCAGTGAATGTCT TACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGACA GGAGGCCCAGAATGATGCCATGATTTTATATGACAAGTACTTTTCCCTCCAAGCCACACACCCCCTTGGA TTTGATGATGTTGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTT TCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGGTCTTTTTGCCTGGTTTTCTGTCCAGCAA TCTTTATTACAAATATTTGAATGATCTCATCCATTCAGTTCGAGGAGATGAATTTCTTGGAGGGAATGTT TCCCTGGCTGCTCACGGCTCTGTCTGCCTTCCTGAGGAGTCTCACTCAGGTGGTTCCGATGGCTCCACTG CTCAGTCTAGTGTGAAAAAAGCCAGTATTAAAATTCTGAAAAATTTTGATGAAGCAATAATTGTGGATGC TGCAAGTCTGGACCCAGAATCTTTATATCAACGGACATATGCAGGGAAGATGTCCTTTGGGAGAGTTAGT GATTTGGGGCAGTTCATCCGAGAGTCTGAGCCTGAACCTGATGTGAAGAAATCAAAAGGATTTATGTTCT CACAAGCTATGAAGAAGTGGGTGCAAGGAAATACTGACGAGGCCCAAGAAGAGCTAGCTTGGAAGATTGC AAAAATGATAGTGAGTGATGTTATGCAGCCGGCACACCATGATCAACCACTAGAGAAGTCTACAAAGCTA TGACTCGAAACCAGAGATAAGGAAATCTGATTTTGAAAAAAATAAGAACTTTCCCCTTTGGTTGGATTCT TCAACACAGCCGATGAAAACGGAGCACTGCATTTCTTTTCACTGCTAGATCACTGTTGTTTCCAGGAAAG AGTGGGAGACAGTCCGAGAGTGGTTGTTAAATGTAGACATAGATGACACACATGACAGTAACACAGTGAG TCCCGAGATACAAAGTGGTGGTGGCGACTTATTAGACCATTTTCCAGTGGCCACGCTCACTGGGGTAGAC AGGGACCACATGCTCGCAGCAGATTACTTCCTCAGCTGTGTCAGAAAGCATGACAGAGGTGCTTCATTCT TGTCTCCTAGAGGTCACAGGCGAGTCAGCCCTGCCTGGAGCGAGCATCTCCAAGTGGCAGTGTGGGCCAG CAGCCTGTGGAGCAGAGGACAATTCCTTGCCTAGTCTTCCTTCACAGAAGCATTAATTTAGAAAGCTGGC AAGTCGTGGGTCAAGCCTCAGTGTTGCATCCTTTCCTTCGCTGACAGAACACACTGTTCACCTTTCCTTC ACTCCCGAGGTGATCGATCCTTAAAGCTAGTCCTTCCTCTGCTGACAAGAGGGCGAGTTTAGTTTACTTT GAGCATCAAGACCCATTCCGCTATCAGAACGCTATGCTCCAGACGCTTCATTCCCTCTTCAGAATACACA TGGAAAAAATGAGCACTCCAGTTTTTCCCTGTGGTCCTTAGAACACATTCACAAAATTGGTTTTATTTTT TAATAAGGTGGGGACAGAAGCACTCCCTTGTGGGTGTCTCCCCAATTCTTCACTGTGAGTGGGAAATGTG CAGTTGCCTTTTCCCTCCCTGTCTTAAGACAGTAGGAGACTCACTCGCTGCCCTTCTGCTTAGTTGGCTT TGCACTTGTGGCACAGCCGTTGGCGGGGCCACCCCGCTGGCAAGCACACTCCCCCAGCCTCACGGTGCCA GTGCTTCCTTGTGTGTAACAGCTTAGAGAGAAAGGAAGTTTTCTTAGGTGTTATTGCTTTTGCTCAGAAT TTGCAAAAGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATATACACATGTACATATATAATTATTA ATCACCTCTGTCCTTGGGGAAAGTCTTGAATGAACAGAAAATTATTTCATTGCAATATTGATTGTACAGA GGCACACTGTTCAACAGAAGAAGCAAAAAGGCTGTGTATAAGTTTTTGGTACTATGTACCATCTCTGTTA TTCTGAAGTATTCATGTACTTAAACCATATTCTATTTAATTGTGTTTGATTTTAAATATATATATATATG AATTATATTTAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30022728 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30022728 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004945 same experiment
	EMAGE:32054 same embryo
	EMAGE:31598 same embryo
	EMAGE:30640 same embryo
	EMAGE:30986 same embryo
	EMAGE:31995 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source