EMAGE mouse gene expression spatial database

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EMAGE:8173

Spry4 sprouty homolog 4 (Drosophila) ( MGI:1345144)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005250. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005250_01	euxassay_005250_02	euxassay_005250_03	euxassay_005250_04

euxassay_005250_05	euxassay_005250_06	euxassay_005250_07	euxassay_005250_08	euxassay_005250_09

euxassay_005250_10	euxassay_005250_11	euxassay_005250_12	euxassay_005250_13	euxassay_005250_14

euxassay_005250_15	euxassay_005250_16	euxassay_005250_17	euxassay_005250_18	euxassay_005250_19

euxassay_005250_20	euxassay_005250_21	euxassay_005250_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8173_wholemount_strong.wlz

8173_wholemount_moderate.wlz

8173_wholemount_weak.wlz

8173_wholemount_possible.wlz

8173_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8173_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 15 16 17 18 19 20 21 22 moderate expression: see section 09 10 11 14 weak expression: see section 12

forearm mesenchyme

strong

regional

strong expression: see section 22

hand

strong

regional

strong expression: see section 01 02 03 20 21 22 moderate expression: see section 04

foot

strong

regional

strong expression: see section 05 16 17 moderate expression: see section 02 03 04 weak expression: see section 18

lower leg mesenchyme

strong

regional

strong expression: see section 01 03 19 20 21 22 moderate expression: see section 02

upper leg mesenchyme

strong

regional

strong expression: see section 21

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

submandibular gland primordium mesenchyme

strong

regional

strong expression: see section 06 07 08 09 14 15 16 17

brain

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vibrissa

strong

regional

strong expression: see section 20 moderate expression: see section 03 04 05 19

glossopharyngeal ix ganglion

moderate

spotted

moderate expression: see section 06 07 16 17

trigeminal v ganglion

moderate

spotted

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19 20

vagus x ganglion

moderate

spotted

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

spotted

moderate expression: see section 04 05 06 07 08 15 16 17 18 19

spinal cord

moderate

single cell

moderate expression: see section 09 10 11 12 13 14

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 12 13

cervical ganglion

strong

regional

strong expression: see section 09 10

thoracic ganglion

strong

regional

strong expression: see section 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 07 08 14 15 16

naris

strong

regional

strong expression: see section 10 11 12 13 14

naso-lacrimal duct

strong

regional

strong expression: see section 05 06 07 16 17 moderate expression: see section 03 04 08

viscerocranium

strong

regional

Expression in the turbinate bone.

tongue

strong

regional

strong expression: see section 10 11 12 13 moderate expression: see section 09

stomach

moderate

regional

moderate expression: see section 03 04 05 weak expression: see section 01 02

rectum

moderate

regional

moderate expression: see section 11

midgut

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13

lower jaw incisor

strong

regional

strong expression: see section 10 13 moderate expression: see section 11

lower jaw molar

strong

homogeneous

strong expression: see section 06 17

upper jaw incisor

strong

regional

strong expression: see section 09 10 13 14 moderate expression: see section 11

upper jaw molar

strong

homogeneous

strong expression: see section 05 06 17 18

metanephros

moderate

regional

moderate expression: see section 06 07 14 15

renal cortex

moderate

regional

moderate expression: see section 05

lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

tail mesenchyme

strong

regional

strong expression: see section 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9107
Entity Detected:	Spry4, sprouty homolog 4 (Drosophila) ( MGI:1345144)
Sequence:	sense strand is shown >T9107 CTCCGACTGACGTCTGCGACTTCAACGGCGACTGACCCACTCGGGTTCGGGGATTTACACAGACGTGGAG CGATGCTTGTGACTCTGCAGCTCCTCAAAGACCCCTAGAAGCCTGTCCCTTGGTGCAGTTCAGGACCTCC AGCCTCATGGAGCCCCCGGTTCCACAGAGCAGCGTCCCTGTGAATCCCAGCTCAGTCATGGTGCAGCCCC TCCTTGACAGCAGAGCGCCCCACAGCCGGCTCCAGCACCCACTCACCATCTTACCCATTGACCAGATGAA GACCAGCCACGTGGAGAATGACTACATAGACAACCCTAGCCTGGCCCCTGCCACGGGTCCCAAGCGGCCC CGGGGCGGGCCACCAGAGCTGGCTCCTACACCTGCCCGCTGTGACCAGGATATTACTCACCACTGGATCT CCTTCAGCGGTCGGCCCAGCTCTGTAAGCAGCAGTAGCAGCACTTCCTCCGACCAGAGGCTCCTAGATCA CATGGCTCCACCGCCCGTGGCTGAGCAGGCCTCACCCAGGGCTGTGCGCCTCCAGCCCAAGGTGGTTCAC TGTAAGCCGCTGGACCTCAAAGGCCCGACAGCTCCGCCAGAGCTAGACAAGCACTTCTTGCTGTGTGAGG CCTGTGGAAAGTGTAAGTGCAAAGAGTGTGCGTCCCCTCGGACGTTACCTTCCTGCTGGGTCTGCAACCA GGAGTGCCTGTGCTCGGCTCAGACGCTGGTCAACTATGGCACATGCATGTGTCTGGTGCAAGGTATCTTC TATCACTGTACTAATGAGGATGATGAGGGCTCTTGCGCCGACCACCCCTGCTCCTGTTCCGGCTCCAACT GCTGCGCCCGCTGGTCCTTCATGGGCGCCCTCTCTGTGGTGCTGCCCTGTCTGCTGTGCTACCTGCCGGC CACAGGCTGCGTCAAGCTGGCCCAGCGAGGCTACGACCGCCTGAGACGCCCCGGTTGCCGCTGCAAGCAC ACGAACAGCGTCATCTGCAAGGCAGCCAGTGGGGACACCAAGACCAGCAGGTCTGACAAGCCTTTCTGAC ACTTTGGATGAAAAAAACATGGTCCCTCCTGCCAGTCTCCTGAGACTGACCTTGCTCATCTGTCCTCTCC TAAAACCCTAGATTGGGAAGAAACCGGCAGAGAACACTACTGCCTAGCCTTGAGTCCCCAAAAGGATGAA GATGTACTTTGGGGATTTTCACAGTCTTGAAACTTTGTTAAACAGCAGTGACAGTGTTAGGGTATGGTGG TAGGGGGCCCAGTTTTCCTATTTCAGAAGACGGAAGACAGATGCGGACACAGGGTCTGCAAGTTCAGCCA CTCAATGGCCTTCCCAGCCCCTCTGCTTCCTCTCATTTTGCCATTCTCTCTGGCTCCTGCTGGAGCCTTC TACCTGGGAGGAACAGCTAATGGGAGGCAAGATACCTCGAGGAAGACCCTCCGAGCCTTGACTTCTATCT TTGCCGCCTCCGTCCATGCCTGTTGGCCTTGGCCTCTTGGGAAGGTGCACCGTAGCCATTCCTGCTGTAT GCACTTGATGTCCGAGAACAAAATCGCCGGTAAGCTGCTGCCTCCCGTGAGTAGCAGGATGGAGAAGGAA CCACCTGGCAGTGTGTTCCTCCTAGCTTTCAATCACTAACTTGGGGGTTGTCCGCTCATCACGCCATCCT CCAGTTCACAGACTGGCCTGCCTGCTATGCGTCCTGAGCACTCTTTCTACTCGGATTCTTTGTAGAAACT CGCTGGGTTAAGAAGGCCAGGATGACACAAGCAAGACCAAATATCATTTTGCCAATGAGTCTGAGGCTGT GGTCTCTGGATCCAGCCATGATGTTTTTATATGTTTAATTAAACTAGATGCTATTGGTGTTTAAACAATA ATAATACAACAACCTTGCTTCCTTTTGGGCCACCCCCAGGAAGGGCTGATTTCAAAATCTGGGGGTAAGC AACCTCAAGGAGCGTTTCCCCTGCACATCGAGAATGGCAAAGAAGAGGCCGTACCTCCCATTGGCAGGAT GACAGTTGAGCCGAACTGGGGTTGGGTTTTCTTGTGATTCTGCTGCTTGCTGTCTCTAGCCTTTTGTGTA TAGTGATGCGTCTGTCTTTATGTAAATAGAGATGACAGAGTCTATTTTAGTGTTAAGAAGCCCTGGTGGG GAAGTCGGAAGATGCCAGAGAAGGTGGGGAACGATCCTCCAGGGGCTGCTGGGTTTGAGCCCCACTTTTT GTGTGCACACACACAGACACCCCTCCCAACAGCCCCCAAAGACGTAGCAACTCTTTCTCTCACGGTGCTA AAGGACTCAGAACGCGCAGCCCGTCCAGTGGGTAGGTACTTGTTGCATGCAAAAAGCTATCGTGTCTCTG GTCCTTGCCCCCAGCTGTTGTGTGGGTGGGTTTTTTTTGTTTTTTTTGTTTTTTTTTTTGTTGTTGTTTT TGGTTTTGGTTTTTTCTTTGTGTGGTATTTTGTCCCCATCCCTGCCTCTGATTCAAGAAATAGCCTGGGT CAGAAGAGGTACCCCAGGTAAAATTGAGAGAGCATTAGGCAAAAATCATGTGGAGCATTTCTGGTTCTTT TGTGTGAGCAGCCCCTAGGGAGGAGGCAAGGAGTCAGGTAGCTGTAAGTGGAGTGTACCTATTGCTCTTT TCTCAAGGATTCACCCCCACCCCCACCTTGGAAGCTACTAGTAGTAATAGTAACACCTTATGATTTAGAA TAAGTTAATTGTAACCATAGGTATTTATTGATTGGAGGAAGGGAGGGTTTTATTTTCAGCTTTATTTATG CAACATTTACTGGCTTGTAAAAGGCAAGTATAAAATCCTGCATCTTCATCGTCTATAACTAATCCATACA TATACCTGCCCTTGCCACATTGCAGTGTTCTTGCACAGAACGGAAGGAAAGATAGAAGACAAACCCCTGC GATCAGCAAACCACTTACACTAATTGAATGTATTAGAAGCTTTCTGAAGGGGCTGGAGATGTTTTCCTCG AATGAGGGGTGTGGAGGGACCCCCCCCCCAAAAAAAAACCCTGATAGCGCACACGTGCACTCTTTCTAAG AAGCCTGGGAGCTTCACCCAGGGTCCCTCCCTCAATTTGTATCCACAGGACAGCTGACCCAGTGGCAGGT TGCACTTTGGTGATCTTGGTCTGCATACGTCTGTGGACAGTTGAGCTATATAAGCCGTGTATGCGCACAT GCACACGTGACACTTACATACACAGACCCTCTACCACACAGACCCTGTTTCCTGGCAACCCTTCCCAAAC CCTGACTTTGTGTACATAGCTGTAAACACGGATTTGGGGGGTTTTGGTTTGCTTCCCCACCTCACCAACC CCTTGTTCTGGTTTGGAAATGATGTCTACAGAATTCTTCCTCCCCACAGAAGTTGACCTTGGGACTCACT TGCTCAGTGGTAAGGGAAGGGGAGAAGGAAGGAAGGAAGCATCCTTTTTCCTCTGCTCTGTTGTTTTTTC TCTGACTTCTTGGCAAACACACATAGGGGATGCAAACACAGAAAAGGGGTAGTTTGTTTATAGGGCCCCC TCCCCTTCCCAGGTTAGTCAACAGGCATTGGGAACACAGGCTCTCACAAACAGAACATTTCTGTAGCTGT TTCTGACTTCTTGGCTAGCATTTGCAGCTTTTGCTTGGCTTGAGTTAACATCACTTCCAAAGTGCCAGCT GGTTCCCACTTCTAGACCTGGGGTGGCTGTCAGTTGTGCCTGGAGCCAAGGATGGCTTCCGGGATCTTTC TTAAGGTGCCTGGGCTACTTGGTACCATTGTAGGGAAAGGCTGGTTCTCCTGTAAGCACTCTGGGGGCTG AGTTTAGGCTAGCAACATCTTCCAAGGTACCATCAATGCTCCTCAAGCCAACTGGAAGAGAGCAACGAGC GGCCTTTCTCAGCGATGGGAGGCATCCTTACGACTGATAGGTTCTTTGAGCTAGTGTAGTTCTGCATCAA TGGGGCCCAGCTGGATCACAGCCTCAAGGGAACAACACATACCAAGTTGGTCAGGGGCAGAACCAGGCAG GTACCCATCATTCTAGAGCAGCTTGGTCTCCAGTGCTGCTCCAAGAAATAGTGGAGGCGTGTGGGCAGCA CACATTTGCACTGTACAATTGAACATCCGGGGCTTCCCCGGCTTCCCAATTTATGCATTTGGTGAGTAGC TCCGTGCCCTTCCCCGCTCTGTAATGGTGGATGTCGATCCTGTGTTTCCATTTTGATTGTGGGTGACTGT GCTGTTTCTTAGCTTGCTTTTCTCTTGTGTCCTGTGGTTGATTTCCTGTTAGCAGCACTAGAGCTGTGCC CAGCACCCCACTCCACCACATTCCTGTGTCTCTGTAGCTCCCCCTCCCCGCCCCTGTTCATCCCACCACA AAAGTTACCACAGAAGGTTTCCTTTGTAAAAAATATTTATTTTGAAGCTCTATTTTAATAGTATTTATTT TAGAAAGTCTACTATTGTAAGAGTTCTTCTGTTTGTGAAGAAAAAAAAACAAGTTAAAAACTGAATGTAC TGATCTAGAAGATATCTATAAATATATATTGTTAAATATAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6821944 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6821944 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8178 same embryo
	EMAGE:8177 same embryo
	EMAGE:8174 same embryo
	EMAGE:8176 same embryo
	EMAGE:8175 same embryo
	EurExpress:euxassay_005250 same experiment
	MGI:4828433 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source